+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Bovine coronavirus structural proteins



Bovine coronavirus structural proteins



Journal of Virology 42(2): 700-707



The tissue culture-adapted strain (Mebus) of bovine coronavirus was grown in the presence of isotopically labeled amino acids, glucosamine, or orthophosphate for the purpose of analyzing the virion structural proteins. Five species of polypeptides were identified when purified virions were solubilized in urea and sodium dodecyl sulfate and resolved by polyacrylamide gel electrophoresis. Four species were glycosylated and had apparent molecular weights of 140,000, 120,000, 100,000, and 26,000. The glycoproteins were susceptible to proteolytic cleavage and enzymatic iodination when intact virions were studied and are thus at least partially external to the virion envelope. The 140,000-molecular-weight glycoprotein is apparently a dimer of 65,000-molecular-weight glycopolypeptides held together by disulfide linkages. Species 5 was phosphorylated and had an apparent molecular weight of 52,000. In the intact virion, it was unaffected by protease and was not enzymatically iodinated. It is therefore apparently an internal protein.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 000831259

Download citation: RISBibTeXText

PMID: 7086972


Related references

Bovine coronavirus structural proteins. Abstracts Of The Annual Meeting Of The American Society For Microbiology: 79, 1979

Antigenic relationships among proteins of bovine coronavirus, human respiratory coronavirus OC43, and mouse hepatitis coronavirus A59. Journal of Virology 51(2): 384-388, 1984

Structural proteins of bovine coronavirus and their intracellular processing. Journal of General Virology 68: 2863-2877, 1987

Structural proteins of bovine coronavirus strain L 9: effects of the host cell and trypsin treatment. Archives of Virology 103(1-2): 35-45, 1988

Genomic relationship of porcine hemagglutinating encephalomyelitis virus to bovine coronavirus and human coronavirus OC43 as studied by the use of bovine coronavirus S gene-specific probes. Archives of virology40(7): 1215-1223, 1995

Mucosal and systemic isotype-specific antibody responses to bovine coronavirus structural proteins in naturally infected dairy calves. American Journal of Veterinary Research 52(6): 852-857, 1991

Characterization of the nonstructural and spike proteins of the human respiratory coronavirus OC43: comparison with bovine enteric coronavirus. Advances in Experimental Medicine and Biology 342: 61-67, 1993

Mucosal and systemic antibody responses to bovine coronavirus structural proteins in experimentally challenge-exposed calves fed low or high amounts of colostral antibodies. American Journal of Veterinary Research 52(5): 700-708, 1991

Isotype-specific antibody responses to bovine coronavirus structural proteins in serum, feces, and mucosal secretions from experimentally challenge-exposed colostrum-deprived calves. American Journal of Veterinary Research 52(5): 692-699, 1991

Cryo-EM structure of infectious bronchitis coronavirus spike protein reveals structural and functional evolution of coronavirus spike proteins. Plos Pathogens 14(4): E1007009, 2018

Canine Respiratory Coronavirus, Bovine Coronavirus, and Human Coronavirus OC43: Receptors and Attachment Factors. Viruses 11(4):, 2019

Phylogenetic analysis and sequence comparisons of structural and non-structural SARS coronavirus proteins in Taiwan. Infection Genetics and Evolution 5(3): 261-269, 2005

Evaluation of the effect of serum antibody abundance against bovine coronavirus on bovine coronavirus shedding and risk of respiratory tract disease in beef calves from birth through the first five weeks in a feedlot. American Journal of Veterinary Research 78(9): 1065-1076, 2017

Multivalent bovine coronavirus vaccine and method of treating bovine coronavirus infection. Official Gazette of the United States Patent & Trademark Office Patents 1223(5), 1999

First detection of bovine coronavirus in Yak (Bos grunniens) and a bovine coronavirus genome with a recombinant HE gene. Journal of General Virology 100(5): 793-803, 2019