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Control of yeast contamination with 5-fluorocytosine in the in vitro cultivation of Leishmania sp



Control of yeast contamination with 5-fluorocytosine in the in vitro cultivation of Leishmania sp



Annals of Tropical Medicine and Parasitology 75(4): 453-454



No significant inhibition of growth of Leishmania promastigotes in vitro occurred using 10 to 625 mu g/ml of 5-fluorocytosine (5-FC) or 250 mu g/ml gentamycin. Tissue scrapings from 'wet' nasal lesions of hamsters infected with a L. major-like parasite (Hafuf 6) were collected, homogenised and inoculated into culture media containing 500 mu g/ml 5-FC and 250 mu g/ml gentamycin, or into control media without 5-FC and gentamycin. Yeasts and bacteria appeared in control media, whereas media containing 5-FC and gentamycin produced good growths of promastigotes free of these contaminants. Promastigotes could be recovered from media contaminated with yeast and fungi, although excessive contamination inhibited promastigote growth.

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Accession: 000848119

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PMID: 7030240


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