+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Control of yeast contamination with 5-fluorocytosine in the in vitro cultivation of Leishmania sp

Control of yeast contamination with 5-fluorocytosine in the in vitro cultivation of Leishmania sp

Annals of Tropical Medicine and Parasitology 75(4): 453-454

No significant inhibition of growth of Leishmania promastigotes in vitro occurred using 10 to 625 mu g/ml of 5-fluorocytosine (5-FC) or 250 mu g/ml gentamycin. Tissue scrapings from 'wet' nasal lesions of hamsters infected with a L. major-like parasite (Hafuf 6) were collected, homogenised and inoculated into culture media containing 500 mu g/ml 5-FC and 250 mu g/ml gentamycin, or into control media without 5-FC and gentamycin. Yeasts and bacteria appeared in control media, whereas media containing 5-FC and gentamycin produced good growths of promastigotes free of these contaminants. Promastigotes could be recovered from media contaminated with yeast and fungi, although excessive contamination inhibited promastigote growth.

(PDF emailed within 1 workday: $29.90)

Accession: 000848119

Download citation: RISBibTeXText

PMID: 7030240

Related references

Elimination of yeast contamination from malaria cultures using 5-fluorocytosine. Annals of Tropical Medicine and Parasitology 74(5): 559-559, 1980

Synergic effect of 5-fluorocytosine and imidazole derivatives against yeast strains resistant to 5-fluorocytosine. Annales de Biologie Clinique 47(9): 546-549, 1989

The effect of human urine on in vitro cultivation of Leishmania infantum and Leishmania tropica isolated in Turkey. Acta Parasitologica Turcica 21(2): 119-121, 1997

In vitro susceptibility to 5-fluorocytosine and nystatin of common clinical yeast isolates. Mycopathologia 73(1): 39-41, 1981

In vitro susceptibility of 119 yeast isolates to fluconazole, 5-fluorocytosine, amphotericin B and ketoconazole. ChemoTherapy 37(1): 23-31, 1991

In vitro susceptibility of 245 yeast isolates to amphotericin B, 5-fluorocytosine, ketoconazole, fluconazole and itraconazole. ChemoTherapy 38(5): 335-339, 1992

In vitro susceptibility of mycelial and yeast forms of Penicillium marneffei to amphotericin B, fluconazole, 5-fluorocytosine and itraconazole. European Journal of Epidemiology 9(5): 553-558, 1993

Removal of bacterial and yeast contamination from Leishmania promastigote cultures, by agar plating. Annals of Tropical Medicine and Parasitology 99(6): 617-621, 2005

Investigation of Routes of Salmonella Contamination Via Soils and the Use of Mulch for Contamination Control during Lettuce Cultivation. Food Science and Technology Research 20(5): 961-969, 2014

Agar barrier method for removal of yeast and other fungal contamination from Leishmania promastigotes culture. Pathogens and Global Health 106(7): 418-420, 2013

Cultivation of Leishmania in vitro. Giorn Batteriol E Immunol 22(6): 881-890, 1939

Distinct Macrophage Fates after in vitro Infection with Different Species of Leishmania: Induction of Apoptosis by Leishmania (Leishmania) amazonensis, but Not by Leishmania (Viannia) guyanensis. Plos One 10(10): E0141196, 2016

Resistance In Vitro of Leishmania Donovani to Contamination with Bacteria. Experimental Biology and Medicine 32(7): 1010-1012, 1935

Peptone-yeast autolysate-fetal bovine serum 10, a simple, inexpensive liquid medium for cultivation of Leishmania spp. Journal of Clinical Microbiology 15(5): 949-950, 1982

Inhibition of growth of Leishmania mexicana mexicana by Leishmania mexicana amazonensis during "in vitro" co-cultivation. Memorias do Instituto Oswaldo Cruz 82(4): 537-542, 1987