Section 1
Chapter 855

Determination of free amino acids by high performance liquid chromatography

Schuster, R.

Analytical Chemistry 52(4): 617-620


ISSN/ISBN: 0003-2700
PMID: 7396199
DOI: 10.1021/ac50054a005
Accession: 000854642

A method for rapid quantitative determination of free amino acids and several vitamins in i.v. solutions and beverages was developed. The separation was performed on a NH2 column with an acetonitrile/phosphate buffer gradient. The samples, injected without a derivatization procedure, were separated within 30 min. With a time programmable wavelength detector, the required high sensitivity was achieved. The relative SD for quantitative results were 1-2%. Quality and stability testing of i.v. solutions are immediate applications of this method.

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