Esterase-16 (Es-16) : characterization, polymorphism, and linkage to chromosome 3 of a kidney esterase locus of the house mouse

von Deimling, O.H.; Schupp, P.; Otto, J.

Biochemical Genetics 19(11-12): 1091-1099

1981


ISSN/ISBN: 0006-2928
PMID: 7337689
DOI: 10.1007/bf00484567
Accession: 000880759

Download citation:  
Text
  |  
BibTeX
  |  
RIS

Article/Abstract emailed within 0-6 h
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Abstract
A polymorphism for an isozyme of a presumed arylesterase, esterase-16 (EC 3.1.1.2), has been detected in kidney, heart, and spleen of the house mouse, Mus musculus, by means of isoelectric focusing and by disc electrophoresis. Three phenotypes can be distinquished: the ES-16A phenotype (IEP 5.9) was found in C57BL/10Sn and many other laboratory inbred strains; the ES-16B phenotype (IEP 6.1) was found in M. m. molossinus; and the ES-16C phenotype (IEP 5.9; very weak activity) was found in Peru-Coppock. Esterase-16 is strongly inhibited by 10(-3) M p-chloromercuribenzoate, but not by 2. 10(-4) M bis-p-nitrophenyl phosphate or by 10(-3) M Diamox. It stains well with indoxyl acetate and other indigogenic substrates but only weakly with alpha-naphthyl acetate. Esterase-16 is completely insoluble in water. It is apparently governed by a structural gene locus, Es-16, with three alleles, Es-16a, Es-16b, and ES-16c, respectively. Es-16 is closely linked to Car-1 and Car-2 on chromosome 3 Typing of 94 animals of the backcross (C57BL/10Sn x M. m. mol.) F1 x M. m. mol. revealed a recombination frequency of 8.51 /+- 2.9%.

Esterase-16 (Es-16): characterization, polymorphism, and linkage to chromosome 3 of a kidney esterase locus of the house mouse