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Feulgen-DNA content and acid lability of nuclei from in vivo and in vitro normal, wound and crown gall tissue of Nicotiana tabacum L



Feulgen-DNA content and acid lability of nuclei from in vivo and in vitro normal, wound and crown gall tissue of Nicotiana tabacum L



Caryologia 33(2): 151-176



Changes in nuclear DNA were studied by measuring the Feulgen DNA content of 8850 nuclei with a scanning and integrating microdensitometer. In in vivo tissues nuclei of young tumor cells [caused by Agrobacterium tumefaciens infection] were more susceptible to hydrolysis (1 N HCl, 60.degree. C) than nuclei of normal, wound and aged tumor cells. In in vitro tissues the hydrolysis curves of callus and tumor nuclei ran parallel; there were no significant differences between young and mature tissues; nuclei from tumor cells stained significantly less than those from callus cells. Aneuploidy or differences in the degree of chromatin condensation are a less likely explanation for this phenomenon. It may be correlated with the state of DNA replication (less protection by histones). No evidence was found for a correlation between tumoral transformation and DNA content or chromosome number, or that tumor tissues would have a greater tendency for polyploidy than callus cells.

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Accession: 000888755

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DOI: 10.1080/00087114.1980.10796828


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