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Immunosorbent electron microscopy for detecting apple chlorotic leaf spot and plum pox viruses



Immunosorbent electron microscopy for detecting apple chlorotic leaf spot and plum pox viruses



Phytopathology 71(4): 400-404



These viruses were detected in extracts of leaves from infected trees by ISEM. Attachment of virus particles to antiserum-coated electron microscope grids provided a test as sensitive as the enzyme-linked immunosorbent assay (ELISA). Confirmation of the identity of the bound particles was obtained by coating further with homologous antibody followed by sheep antirabbit immunoglobulins which increases the sensitivity and facilitates the interpretation of the test.

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Accession: 000906148

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