+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Nucleotide sequence homology of thirteen tobamovirus RNAs as determined by hybridization analysis with complementary DNA



Nucleotide sequence homology of thirteen tobamovirus RNAs as determined by hybridization analysis with complementary DNA



Virology 107(2): 354-361



The extent of RNA sequence homology between 13 isolates of tobamoviruses has been estimated by hybridization analysis using radioactive complementary DNA. Hybrid formation between complementary [3H]DNA prepared against 7 of the tobamovirus RNAs and the RNAs of the 13 isolates was assayed with the single-strand-specific S1 nuclease. The presence of mismatched regions in the DNA: RNA hybrids was shown by variation of the stringency of the conditions for the hybridization and S1 nuclease assay. The data obtained have allowed the allocation of the 13 isolates into five groups on the basis of significant sequence homology between members of each group. Members of two of these groups were further divided into subgroups on the basis of differences in the hybridization data. Although hybridization analysis using labeled complementary DNA is considered the method of choice at present for estimating sequence homology between viral RNAs, the limitations inherent in any hybridization approach are discussed.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 000938692

Download citation: RISBibTeXText

PMID: 18631835

DOI: 10.1016/0042-6822(80)90303-7


Related references

Determination of the sequence homology between the four RNA species of cucumber mosaic virus by hybridization analysis with complementary DNA. Nucleic Acids Research 4(11): 3787-3802, 1977

Partial amino acid sequence of human thrombospondin as determined by analysis of complementary dna clones homology to malarial circumsporozoite proteins. Biochemistry 25(26): 8418-8425, 1986

The use of hybridization analysis with complementary DNA to determine the RNA sequence homology between strains of plant viruses: its application to several strains of cucumoviruses. Virology 88(2): 361-370, 1978

An assessment by competition hybridization of the sequence homology between the RNAs of the seven serotypes of FMDV. Journal of General Virology 37(2): 271-276, 1977

Comparison of the genome RNA sequence homology between cricket paralysis virus and strains of Drosophila C virus by complementary DNA hybridization analysis. Journal of general virology 65(7): 1193-1196, 1984

Amino acid sequence of silkworm bombyx mori hemolymph antitrypsin deduced from its complementary dna nucleotide sequence confirmation of its homology with serpins. Journal of Biochemistry (Tokyo) 108(3): 372-378, 1990

Inter species sequence conservation of guanine nucleotide binding protein complementary dna in coding and untranslated regions determined by lambda gt and complementary dna amplification. Clinical Research 37(2): 609A, 1989

Noncoding nucleotide sequence in the 3 prime terminal region of a mouse immuno globulin kappa chain messenger rna determined by analysis of complementary dna. Biochemistry 16(18): 4117-4125, 1977

Measurement of viroid sequence homology by hybridization with complementary DNA prepared in vitro. Virology 89(2): 388-394, 1978

The nucleotide sequences of low molecular weight ribosomal rnas from different materials and comparison of the sequence homology. Kon, Ot Al. (ed.). Icsu (international Council Of Scientific Unions) Short Reports, Vol. 6. Contemporary Themes In Biochemistry; 4th Federation Of Asian Oceanian Biochemists Congress, Singapore, November 30-December 5, . Xxxvii 715p. Cambridge University Press: Cambridge, England, Uk; New York, New York, Usa. Illus. 180-181, 1986, 1987

Nucleotide sequence of the 3'-noncoding region of alfalfa mosaic virus RNA 4 and its homology with the genomic RNAs. Nucleic Acids Research 7(7): 1887-1900, 1979

Nucleotide sequence of cucumber mosaic virus RNA. 1. Presence of a sequence complementary to part of the viral satellite RNA and homologies with other viral RNAs. European Journal of Biochemistry 150(2): 331-339, 1985

A 43-base-pair complementary DNA sequence homology and triplet repeat motif among putative polymeric immunoglobulin receptor messenger RNAs in regenerating rat liver. Hepatology 18(1): 226-228, 1993

Homology among eleven flavivirus by comparative nucleotide sequence of genomic rnas and deduced amino acid sequences of viral proteins. Tropical Medicine 33(1-2): 23-33, 1991

Nucleotide sequence of a complementary dna encoding bovine brown fat uncoupling protein homology with adp binding site of adp atp carrier. Nucleic Acids Research 17(5): 2131, 1989