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Regulation of gene expression in corn (Zea mays L.) by heat shock


, : Regulation of gene expression in corn (Zea mays L.) by heat shock. Canadian Journal of Biochemistry 60(5): 569-579

Subjecting 5-day old plumules of corn (Z. mays L.) to elevated temperatures of brief periods of time causes the pattern of protein synthesis to shift from the production of a broad spectrum of proteins to the new and (or) enhanced synthesis of a small number of heat-shock polypeptides (HSPs). Most notable is the depressed synthesis of a major polypeptide (relative mass (Mr) =93,000 and isoelectric point = 8.0) normally made at 27.degree. C and the enhanced and (or) new synthesis of polypeptides with Mr of 108,000, 89,000, 84,000, 76,000, 73,000 and 18,000, following 1 h of heat shock. These 6 HSPs resolve into 18 spots by 2-dimensional fluorographic analysis. The induction of the HSPs requires temperatures at or exceeding 35.degree. C for detectable synthesis. Some of the HSPs are synthesized following only 15 min at 41.degree. C and synthesis of all HSPs is observed within 120 min following heat shock. Recovery from heat shock is rapid; after 6-8 h at 27.degree. C following heat shock, the polypeptide pattern is indistinguishable from the control. Extracts from individual heat-shocked shoots produced polypeptide synthetic patterns identical to those from extracts from 20 shoots, regardless of whether single shoots were intact or excised during labeling. Single 5-day old primary roots exhibited polypeptide synthetic patterns and responded to heat shock in a manner similar to shoots. This is the 1st demonstration of the induction of heat-shock polypeptides in a whole, intact higher plant.

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Accession: 000966161

PMID: 7104834

DOI: 10.1139/o82-070

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Beller, K., 1939: Diarrhoeic Diseases of Young Animals Compared with Similar Disease in Infants. This article is mainly a comprehensive review of diseases in animals (including birds) which result in diarrhoea in the young within a few days or weeks of birth. Some comparisons are made with diarrhoeic diseases of infants. There are over 800 re...

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Stead, J. W., 1983: A study of variation and taxonomy of the Pinus pseudostrobus complex. A study was carried out in order to minimize taxonomic problems in the International Provenance Trials of P. pseudostrobus organized by the Commonwealth Forestry Institute (CFI). Collections of approx. 25 trees were made at each of 18 sites throug...

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