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Resistance of bull spermatozoa to low temperatures: variability of behaviour between ejaculates

Resistance of bull spermatozoa to low temperatures: variability of behaviour between ejaculates

Bulletin de l' Academie Veterinaire de France 54(1): 125-130

20 ejaculates were collected from 20 bulls. 48 semen doses (in 0.25-ml straws, containing 20 X 106 spermatozoa) were prepared from each ejaculate, and were frozen by 8 methods. The methods were: group 1, direct immersion in liquid nitrogen; groups 2-8, immersion in liquid nitrogen after freezing in liquid nitrogen vapour until semen temp. reached -20 deg , -30 deg , -50 deg , -70 deg , -80 deg , -120 deg and -163 deg C resp. Group 8 was the control.

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Accession: 000969767

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