Somatic hybridization between Nicotiana rustica and N. tabacum. IV. Analysis of nuclear and chloroplast genome expression in somatic hybrids

Douglas, G.C.; Wetter, L.R.; Keller, W.A.; Setterfield, G.

Canadian Journal of Botany 59(8): 1509-1513

1981


ISSN/ISBN: 0008-4026
DOI: 10.1139/b81-205
Accession: 000982674

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Abstract
Ribulose 1,5-diphosphate carboxylase/oxygenase was isolated from 14 N. rustica + N. tabacum somatic hybrid plants and was subjected to isoelectric focusing after carboxymethylation. The resulting polypeptide banding pattern for the nuclear-encoded small subunit of the enzyme showed bands derived from both parental species, whereas the banding pattern for the plastid-encoded large subunit showed bands characteristic of either N. rustica or N. tabacum, but not both species. Eleven plants expressed N. tabacum chloroplast genes.