Somatic hybridization between male sterile Nicotiana tabacum and N. glutinosa through protoplast fusion
Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 61(1): 69-72
ISSN/ISBN: 0040-5752 PMID: 24271377 DOI: 10.1007/bf00261514
Protoplasts derived from suspension cultured cells of cytoplasmic male sterile Nicotiana tabacum (N. debneyi cytoplasm) and of fertile N. glutinosa were fused with the aid of polyethylene glycol (PEG). Out of 1,089 colonies developed from PEG-treated protoplasts, 29 restored whole plants.A somatic hybrid plant was selected on the basis of isoelectrofocusing analysis of Fraction I protein in leaves of regenerated plants. A newly created hybrid contained small subunits of both parents but only a N. glutinosa type large subunit.Male sterile character was conserved in a hybrid plant while leaf morphology was intermediate between the parents.