+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

The in vitro effects of serum on the adults, metacercariae, and eggs of Opisthorchis viverrini

The in vitro effects of serum on the adults, metacercariae, and eggs of Opisthorchis viverrini

Transactions of the Royal Society of Tropical Medicine and Hygiene5(6): 825-831

Adult and juvenile Opisthorchis viverrini liver-flukes incubated in hamster chronic infection serum (CIS) developed tegumental precipitates which enclosed the entire parasite. Adult worms appeared dead by 48 hours in CIS and juvenile worms by 18 hours. No such reactions were observed in normal hamster serum (NHS). Heat inactivation of CIS at 56 degrees C abolished the precipitin reactivity against both adult and juvenile parasites though reactivity was regained upon addition of NHS or guinea-pig serum. Reactivity was also abolished by heat inactivating CIS at 60 degrees C but reactivity was not regained upon addition of NHS or guinea-pig serum. Coarse granular precipitates and blebs were observed around eggs in CIS and this activity was abolished by heat inactivating serum at 60 degrees C but not 56 degrees C. Aging of adult parasites in vitro for nine or 27 days before testing against CIS did not affect the development of tegumental precipitates although freeze-thaw killing of adult parasites before testing against CIS resulted in a marked reduction in the extent of the tegumental precipitate reaction. These results show that serum from O. viverrini-infected hamsters contains a component(s), possibly a specific immunoglobulin(s) capable of reacting with adult, juvenile and egg state parasitic antigens. These findings are discussed in relation to immunopathological processes that might be operating in the liver of the infected host.

(PDF emailed within 0-6 h: $19.90)

Accession: 001010288

Download citation: RISBibTeXText

PMID: 7330943

DOI: 10.1016/0035-9203(81)90421-1

Related references

Viable metacercariae of Opisthorchis viverrini in northeastern Thai cyprinid fish dishes--as part of a rational program for control of O. viverrini-associated cholangiocarcinoma. Parasitology Research 112(3): 1323-1327, 2013

Effects of physico-chemical factors on the infection of hamsters by metacercariae of Opisthorchis viverrini. Southeast Asian Journal of Tropical Medicine and Public Health 13(4): 614-617, 1982

Effects of fermentation time and low temperature during the production process of Thai pickled fish (pla-som) on the viability and infectivity of Opisthorchis viverrini metacercariae. International Journal of Food Microbiology 218: 1-5, 2016

A method for collecting metacercariae of Opisthorchis viverrini. Siriraj Hospital Gazette 35(2): 143-146, 1983

Prevalence of Opisthorchis viverrini infection among villagers harboring Opisthorchis-like eggs. Southeast Asian Journal of Tropical Medicine and Public Health 32 Suppl 2: 23-26, 2002

Alcohol and alkalosis enhance excystation of Opisthorchis viverrini metacercariae. Parasitology Research 112(6): 2397-2402, 2013

Discovery of Opisthorchis viverrini metacercariae in freshwater fish in southern Cambodia. Acta Tropica 111(2): 108-113, 2009

Function association of enhanced expressed genes between Opisthorchis viverrini and non Opisthorchis viverrini-related cholangiocarcinoma. Revista Espanola de Enfermedades Digestivas 100(7): 439-440, 2008

Comparison of gene expression profiles between Opisthorchis viverrini and non-Opisthorchis viverrini associated human intrahepatic cholangiocarcinoma. Hepatology 44(4): 1025-1038, 2006

Distribution and abundance of Opisthorchis viverrini metacercariae in cyprinid fish in Northeastern Thailand. Korean Journal of Parasitology 51(6): 703-710, 2014

Infectivity and development of Opisthorchis viverrini metacercariae in immunosuppressed Barbonymus gonionotus fingerlings (Cyprinidae). Acta Tropica 162: 107-113, 2017

Carcinogenic human liver fluke: current status of Opisthorchis viverrini metacercariae in Nakhon Ratchasima, Thailand. Asian Pacific Journal of Cancer Prevention 13(4): 1235-1240, 2013

New locality record for Haplorchoides mehrai and possible interactions with Opisthorchis viverrini metacercariae in cyprinid fishes in Northeast Thailand. Parasitology Research (): -, 2016

Immunization and challenge shown by hamsters infected with Opisthorchis viverrini following exposure to gamma-irradiated metacercariae of this carcinogenic liver fluke. Journal of Helminthology 90(1): 39-47, 2016