+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Variable and common antigens of Babesia bovis parasites differing in strain and virulence



Variable and common antigens of Babesia bovis parasites differing in strain and virulence



Journal of Immunology 129(4): 1700-1705



Virulent and avirulent K strain (KV and KA) and virulent L strain (LV) Babesia bovis parasites were biosynthetically labeled with 35S-methionine. Labeled proteins, extracted in detergent from infected cultures, were analyzed by two-dimensional gel electrophoresis. Protein antigens common to these forms of babesia and those specific for strain and correlating with the degree of virulence were identified by immunoprecipitation with antisera. Dominant labeled protein antigens were found to differ both between the K and L strains of B. bovis and between virulent (KV) and avirulent (KA) forms of the K strain. Partial cleavage of biosynthetically labeled proteins by staphylococcal V-8 protease and peptide mapping revealed that no extensive amino acid homology existed between the candidate variant antigens. KA B. bovis parasites are currently in use as a live attenuated vaccine for bovine babesiosis in Australia. Antigens that are better expressed in KA relative to virulent strains may therefore be markers of avirulence and/or candidate "host-protective antigens" of B. bovis. At least one protein (Mr 43,000) was identified as being a dominant labeled antigen of KA that is poorly represented in the KV and LV B. bovis isolates.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 001030224

Download citation: RISBibTeXText

PMID: 7108225


Related references

Babesia bovis transovarian transmission in Boophilus microplus obtention of a Babesia free tick strain Transmissao transovariana de Babesia bovis em Boophilus microplus obtencao de cepa de carrapato livre de Babesia spp. Ciencia Rural. mai-jun; 303: 455-459, 2000

Identification of common antigens in Babesia bovis, B. bigemina, and B. divergens. Annals of the New York Academy of Sciences 1081: 382-396, 2006

Virulence and heterologous strain immunity of South African and Australian Babesia bovis strains with reduced pathogenicity. Onderstepoort Journal of Veterinary Research 49(3): 133-136, 1982

Cross-protective immunity induced by Babesia bovis clones with antigenically unrelated variable merozoite surface antigens. Veterinary Immunology and Immunopathology 41(3-4): 367-374, 1994

Cell-mediated immune responses to Babesia bovis merozoite antigens in cattle following infection with tick-derived or cultured parasites. Infection and Immunity 59(7): 2418-2426, 1991

Attempts to adapt the cattle parasites babesia bovis and babesia bigemina in the syrian hamster. Abstracts of the Annual Meeting of the American Society for Microbiology 90: 75, 1990

Cloning of babesia bigemina and babesia bovis repetitive dna for species specific identification of the parasites. Memorias do Instituto Oswaldo Cruz 85(SUPPL 1): 157, 1990

Detection of tick blood parasites in Egypt using PCR assay I--Babesia bovis and Babesia bigemina. Parasitology Research 105(3): 721-730, 2009

Immunodominant T-cell antigens and epitopes of Babesia bovis and Babesia bigemina. Annals of Tropical Medicine and Parasitology 92(4): 473-482, 1998

Immunochemical characterization of in vitro culture-derived antigens of Babesia bovis and Babesia bigemina. Veterinary Parasitology 76(4): 239-249, 1998

Comparison of different direct diagnostic methods to identify Babesia bovis and Babesia bigemina in animals vaccinated with live attenuated parasites. Veterinary Parasitology 139(1-3): 231-236, 2006

Seroepidemiology of bovine Babesia and Anaplasma infections in Colombia. II. Improved manufacture of antigens for indirect immunofluorescence with Babesia bovis. Seroepidemiologie der bovinen Babesien und Anaplasmeninfektionen in Kolumbien II Verbesserung der Antigenherstellung fur indirekte Immunfluoreszenz mit Babesia bovis: 131, 1984

Attenuation of babesia argentina babesia bovis strain isolated in colombia. Revista del Instituto Colombiano Agropecuario 14(1): 33-40, 1979

Transovarian transmission of Babesia bovis in Boophilus microplus: obtaining a Babesia-free tick strain. Ciencia Rural 30(3): 455-459, 2000

Babesia bovis, Babesia bigemina, Babesia canis, Babesia microti and Babesia rodhaini: comparison of ribosomal RNA gene organization. International Journal for Parasitology. September; 226: 851-855, 1992