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Variant specific glycoproteins of Trypanosoma equiperdum: cross reacting determinants and chemical studies



Variant specific glycoproteins of Trypanosoma equiperdum: cross reacting determinants and chemical studies



Biochemical and Biophysical Research Communications, 992: 729-736



Two variant specific surface antigens (VSSA) (BoTat-1 and BoTat-28) were purified from clones of Trypanosoma equiperdum and tested for immunological cross reactivity. Competitive radioimmunoassay revealed the presence of cross-reacting determinants. Limited tryptic cleavage of BoTat-28 resulted in the purification of 2 fragments F1 and F2 with respectively 38 000 and 28 000 apparent molecular weight. F1 represents a large N-terminal fragment containing 30% of the total sugar content. F2 is characterized by an N-terminal amino acid sequence different from that of the native glycoprotein and containing all the carbohydrate. Inhibition of the heterologous precipitation reaction was only achieved with F2 and reaches 100% as with the native glycoproteins. These results demonstrate the presence of cross-reacting determinants located in the C-terminal part of the molecule. [AS].

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Accession: 001030259

Download citation: RISBibTeXText

PMID: 6165364

DOI: 10.1016/0006-291x(81)91804-0


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