A high humidity incubation chamber for foliar pathogens

Krupinsky J. M.; Scharen A. L.

A chamber used to create and maintain a high humidity atmosphere for inoculations with foliar pathogens of plants is described. The chamber was constructed from polyvinyl chloride pipe, which is inert and rustproof. The clear polyethylene plastic cover on the chamber was replaced easily whenever necessary. Humid air piped into the chamber from 2 commercial cold-water humidifiers was evenly distributed along the sides of the chamber to provide uniform high humidity throughout the chamber. Relative humidity levels of 99 and 100% were maintained at various temperatures ranging 14-36.degree. C inside the chamber.