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A high-pressure liquid chromatographic-enzymatic assay for glycine and taurine conjugates of cholic, chenodeoxycholic and deoxycholic acid in serum



A high-pressure liquid chromatographic-enzymatic assay for glycine and taurine conjugates of cholic, chenodeoxycholic and deoxycholic acid in serum



Scandinavian Journal of Clinical and Laboratory Investigation 42(5): 455-460



A method is described for estimation of glycine and taurine conjugates of cholic, chenodeoxycholic and deoxycholic acid in serum by a high pressure liquid chromatographic-enzymic system. The bile acids are extracted from serum by reverse-phase liquid chromatography with an octadecylsilane column. The bile acid conjugates are separated on a mu Bondapak C18 column with methanol/KH2PO4 buffer, 20 mmol/litre, pH 5.3 as a mobile phase in less than 30 min at a flow rate of 1.4 ml/min.

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Accession: 001038148

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PMID: 7156857

DOI: 10.1080/00365518209168113


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