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A method for collecting metacercariae of Opisthorchis viverrini

A method for collecting metacercariae of Opisthorchis viverrini

Siriraj Hospital Gazette 35(2): 143-146

A 100 g sample of minced cyprinoid fish is incubated with one litre of pepsin A solution at 37 deg C for one h and the resulting solution passed through 300 and 53 mu m sieves. The sediment in the 53 mu m sieve is washed 3 times with normal saline solution, and the washings are spun down in a bench centrifuge and the supernatent discarded.

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Accession: 001038360

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