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A method of inoculating bean with the pathogen of anthracnose and evaluation of the reaction for resistance



A method of inoculating bean with the pathogen of anthracnose and evaluation of the reaction for resistance



Rasteniev"dni Nauki 20(1): 139-148



In studies on inoculation of Phaseolus vulgaris with Colletotrichum lindemuthianum the most suitable medium was glucose-peptone agar at pH 5.95. Sporulation was max. at 20.1 deg C, with an opt. conc. of 106 spores/ml, and the spore suspension could be stored for 37 h at 5 deg or for 6 h at 18 deg. Infection was max. on hypocotyl inoculation 1 day after germination and on keeping the plants in a moist chamber for 96 h.

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