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A modified incubation condition of enzyme-linked immunosorbent assay for detection of watermelon mosaic and cucumber mosaic viruses



A modified incubation condition of enzyme-linked immunosorbent assay for detection of watermelon mosaic and cucumber mosaic viruses



Annals of the Phytopathological Society of Japan 48(2): 192-198



Incubation conditions for the double antibody sandwich method of ELISA were evaluated for the detection of WMV and CMV. When adsorbing specific antibody (globulin) to plate wells, there were no clear differences in ELISA absorbance values for WMV at 3 incubation temps. of 6, 27 and 37 deg C. However, when adding test samples to plate wells, combination incubation of 27 deg for 2 h and 6 deg for 14 h gave max. values for the 2 viruses.

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Accession: 001038578

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