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Bud formation in trapezond tobacco stem explants in culture in vitro under conditions of their contact with explants having a different type of morphogenesis



Bud formation in trapezond tobacco stem explants in culture in vitro under conditions of their contact with explants having a different type of morphogenesis



Doklady Botanical Sciences 265/267: 100-103



Reciprocal influences of explants through an agar nutrient medium and under conditions of contact cultivation or formation of flower and vegetative buds in vitro in day-neutral tobacco Trapezond were studied. formation of flower buds was increased in the explants from flowering plants cultured on a medium where segments of cortex from the inflorescence axis had been cultured earlier; formation of vegetative buds increased on a medium where segments of cortex from the vegetating plants had been cultured. Formation of vegetative buds increased markedly and that of flower buds was not induced in the explants from the vegetative plants when cultured on a medium where segments of cortex from flowering or vegetating plants had been cultured. In another experiment, explants from the inflorescence axis, the middle part of flowering plants and the vegetating plants were superimposed on each other in different combinations and were cultured for 7 days, after which the upper explants were removed and transferred to fresh culture medium. The number of flower buds decreased and that of vegetative buds increased in receptor explants from flowering plants under the influence of explants from vegetating plants. The flower bud formation could not be induced in receptor explants from vegetating plants under the influence of explants from flowering plants. Although explants from the middle part of flowering plants mostly formed the vegetative buds, their capacity to form flower buds was increased under the influence of explants from the inflorescence axis. The results showed the presence of a physiological gradient in different plant parts of tobacco Trapezond.

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Accession: 001050931

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