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Characterization and in vitro synthesis of the 11S globulin from soybean (Glycine max.) seeds


, : Characterization and in vitro synthesis of the 11S globulin from soybean (Glycine max.) seeds. Dissertation Abstracts International, B 41(6): 2004-2005

11S globulin was extracted from soyabean seeds using 2 methods.

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Related references

D.C.stro L.; Breidenbach R.W., 1977: Proteins of soybean seeds part 3 isolation and characterization studies on the iis globulin from soybean seeds. Plant Physiology (Rockville) 59(6 SUPPL): 68

Koshiyama I.; Hamano M.; Fukushima D., 1981: A heat denaturation study of the 11s globulin in soybean glycine max seeds. When the 11S globulin, one of the major storage proteins in soybean seeds (G. max), was heated at 0.5 ionic strength, the denaturation temperature was biologically estimated to be .apprx. 10.degree. higher than that at 0.1 ionic strength. The resu...

Calmes J.; Viala G., 1981: Protein synthesis in soybean glycine max seeds role of photo respiratory glycine and serine. In G. max (L.) Merr, photorespiratory glycine and serine are partially used for protein synthesis in leaves and pods. Glycolate-1-14C feeding, in light or darkness, demonstrated that glycine and serine are translocated to the pods and incorporated...

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Funk, M.O.; Carroll, R.T.; Thompson, J.F.; Dunham, W.R., 1986: The lipoxygenases in developing soybean seeds, their characterization and synthesis in vitro. A number of lipoxygenase isoenzymes were identified in developing soybean (Glycine max L. Merrill cv Provar) seeds and two have been partially characterized. In a study of lipoxygenase level in developing soybean seeds, the enzyme content increase...

Beachy, R.N.; Barton, K.A.; Thompson, J.F.; Madison, J.T., 1980: In vitro synthesis of the alpha and alpha' subunits of the 7s storage proteins conglycinin of soybean glycine max seeds. mRNA, isolated from immature soybean (G. max L., Merr.) seeds, that bound to oligo(dT)-cellulose were fractionated by centrifugation in sucrose density gradients containing dimethyl sulfoxide. mRNA with sedimentation values between 21S and 25S cod...

Harada K.; Toyokawa Y.; Kitamura K., 1983: Genetic analysis of the most acidic 11s globulin subunit and related characters in soybean glycine max seeds. The A5 subunit is the most acidic subunit of soybean 11 S globulin. The inheritance of A5 subunit was studied by analyzing electrophoretically the crude 11 S globulins in single seeds of F1 and F2 of 4 crosses. Some of these seeds were used to inv...

Hayashi, M.; Harada, K.; Fujiwara, T.; Kitamura, K., 1998: Characterization of a 7S globulin-deficient mutant of soybean (Glycine max (L.) Merrill). We attempted to characterize a soybean mutant lacking the 7S globulin (beta-conglycinin) subunits, alpha, alpha' and beta. The results of Southern and northern blot analyses indicated that the deficiency is not caused by a lack of, or structu...

Hayashi, M.; Harada, K.; Fujiwara, T.; Kitamura, K., 1998: Characterization of a 7S globulin-deficient mutant of soybean (Glycine max (L.) Merrill). We attempted to characterize a soybean mutant lacking the 7S globulin (beta-conglycinin) subunits, alpha, alpha' and beta. The results of Southern and northern blot analyses indicated that the deficiency is not caused by a lack of, or structu...

Yasui T., 1985: Dissimilarity in low molecular weight carbohydrate composition of the seeds of cultivated soybean glycine max ssp max and wild soybean glycine max ssp soja. The composition of low molecular weight carbohydrates in 26 samples of cultivated and wild soybeans were determined by GLC. Sucrose and stachyose were major sugars in their seeds. The wild and cultivated types were different in their carbohydrate...