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Characterization and properties of the human and bovine lactotransferrins extracted from the faeces of newborn infants


Acta Paediatrica Scandinavica 71(6): 979-985
Characterization and properties of the human and bovine lactotransferrins extracted from the faeces of newborn infants
Human lactotransferrin was characterized in the fecal extracts from breastfed babies. Quantitative determination of human copro-lactotransferrin from birth up to 3 wk showed that the daily elimination decreased from 35 to 5 mg. The amount of copro-lactotransferrin corresponding to the endogenous secretion was calculated to be 0.5-1 mg/day. When a cow's milk diet supplemented by partially or completely Fe-saturated human or bovine lactotransferrin was fed to the babies, the amounts of copro-lactotransferrin excreted depended on the origin and on the Fe saturation of the lactotransferrin. In particular, the amount of bovine copro-lactotransferrin in the feces averaged 200 mg/day. The human and bovine copro-lactotransferrins were isolated by ion-exchange chromatography or by affinity chromatography and were still able to bind Fe. The fingerprints of native human and bovine lactotransferrins hydrolyzed in vitro by infant's gastric or duodenal secretions showed that both proteins were not extensively digested. This demonstrates that these lactotransferrins ingested by babies are not completely destroyed and keep their ability to bind Fe, and thus may supplement the bacteriostatic effects of the endogenous lactotransferrin in the intestinal tract.


Accession: 001053313

PMID: 6818832



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