EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Characterization of chicken lymphocyte subsets separated by peanut agglutinin


Cellular Immunology 80(2): 288-300
Characterization of chicken lymphocyte subsets separated by peanut agglutinin
The reactivity of chicken lymphocytes isolated from bursa, thymus, spleen and peripheral blood (PBL) with peanut agglutinin (PNA) was investigated. High numbers of cells binding PNA (PNA+ cells) were found in bursa (74.5%) and thymus (85.2%), and in peripheral organs (spleen and PBL, 43.8 and 70%, respectively). In the latter organs, the levels of PNA+ cells exceeded by far the values reported for mammals. Separation of PNA+ and PNA- cells from different organs was achieved by selective agglutination with the lectin or by affinity chromatography on immobilized PNA. Experiments combining the use of limiting concentrations of neuraminidase to cleave off sialic acid from the surface membrane of splenocytes that do not bind PNA (PNA- cells), followed by agglutination with the lectin, suggested that the high binding of PNA to mature lymphocytes is most likely due to a low degree of sialylation of chicken cell surface glycoconjugates. The functional properties in vitro of PNA+ and PNA- spleen cell fractions were assayed in mitogen reponses (PHA [phytohemagglutinin], Con A [concanavalin A], PWM [pokeweed mitogen]), the mixed-lymphocyte reaction (MLR) and the in vitro anti-SRBC [sheep red blood cell] antibody response. In all 3 systems the responses of the PNA+ fraction were significantly higher than those of the PNA- cells. Mixing experiments revealed that irradiated (1500 R) PNA- cells preferably suppress pure T cell responses of unfractionated spleen cells, whereas PNA- cells exerted a stronger suppressive effect on responses involving B cells. In the chicken, PNA may be a valuable tool to distinguish subsets of suppressor cells with different target specificity.

Accession: 001053374

PMID: 6349827

DOI: 10.1016/0008-8749(83)90117-x

Download PDF Full Text: Characterization of chicken lymphocyte subsets separated by peanut agglutinin



Related references

Peanut agglutinin 6. identification of murine t lymphocyte subsets during ontogenesis by use of peanut agglutinin and ly 6.2 anti serum. Journal of Immunology 125(4): 1702-1707, 1980

Peanut agglutinin. VI. Identification of murine T lymphocyte subsets during ontogenesis by use of peanut agglutinin and Ly-6.2 antiserum. Journal of Immunology 125(4): 1702-1707, 1980

Characterization of human umbilical cord blood lymphocyte subsets fractionated on immobilized peanut agglutinin. Human Immunology 7(2): 67-78, 1983

Lymphocyte subsets in human thymus: expression of IgM-Fc receptor by peanut agglutinin positive and negative thymocytes. Thymus 2(4-5): 225-233, 1981

Lymphocyte subsets in human thymus expression of immuno globulin m fc receptor by peanut agglutinin positive and negative thymocytes. Thymus 2(4-5): 225-234, 1981

Peanut agglutinin. II. Characterization of the Thy-1, Tla and Ig phenotype of peanut agglutinin-positive cells in adult, embryonic and nude mice using double immunofluorescence. European Journal of Immunology 9(2): 139-145, 1979

Pre-B cells in mouse bone marrow: in vitro maturation of peanut agglutinin binding B lymphocyte precursors separated from bone marrow by fluorescence-activated cell sorting. Journal of Immunology 133(1): 86-90, 1984

Thymocyte sub populations in guinea pigs 3. physical and functional characterization of 6 sub populations separated by density gradient centrifugation and peanut agglutinin binding. Experimental Cell Biology 51(5): 257-266, 1983

Peanut agglutinin part 2 characterization of the thy 1 tla and immuno globulin phenotype of peanut agglutinin positive cells in adult embryonic and nude mice using double immuno fluorescence. European Journal of Immunology 9(2): 139-145, 1979

Relationship between mouse lymphocyte receptors for peanut agglutinin and helix pomatia agglutinin. European Journal of Immunology 13(10): 831-840, 1983