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Characterization of chicken lymphocyte subsets separated by peanut agglutinin


, : Characterization of chicken lymphocyte subsets separated by peanut agglutinin. Cellular Immunology 80(2): 288-300

The reactivity of chicken lymphocytes isolated from bursa, thymus, spleen and peripheral blood (PBL) with peanut agglutinin (PNA) was investigated. High numbers of cells binding PNA (PNA+ cells) were found in bursa (74.5%) and thymus (85.2%), and in peripheral organs (spleen and PBL, 43.8 and 70%, respectively). In the latter organs, the levels of PNA+ cells exceeded by far the values reported for mammals. Separation of PNA+ and PNA- cells from different organs was achieved by selective agglutination with the lectin or by affinity chromatography on immobilized PNA. Experiments combining the use of limiting concentrations of neuraminidase to cleave off sialic acid from the surface membrane of splenocytes that do not bind PNA (PNA- cells), followed by agglutination with the lectin, suggested that the high binding of PNA to mature lymphocytes is most likely due to a low degree of sialylation of chicken cell surface glycoconjugates. The functional properties in vitro of PNA+ and PNA- spleen cell fractions were assayed in mitogen reponses (PHA [phytohemagglutinin], Con A [concanavalin A], PWM [pokeweed mitogen]), the mixed-lymphocyte reaction (MLR) and the in vitro anti-SRBC [sheep red blood cell] antibody response. In all 3 systems the responses of the PNA+ fraction were significantly higher than those of the PNA- cells. Mixing experiments revealed that irradiated (1500 R) PNA- cells preferably suppress pure T cell responses of unfractionated spleen cells, whereas PNA- cells exerted a stronger suppressive effect on responses involving B cells. In the chicken, PNA may be a valuable tool to distinguish subsets of suppressor cells with different target specificity.

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Accession: 001053374

PMID: 6349827

DOI: 10.1016/0008-8749(83)90117-x

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Hein, J., 1985: Are there mucoviscidosis specific humoral factors? 1: Properties, prevalence, preparation, formation. In 1967 Spock et al. reported on the serum of cystic fibrosis (CF) homozygotes containing a factor altering the coordination of ciliary motion in rabbit tracheal explants. Just in 1967 Mangos et al. found sweat and saliva from CF homozygotes havin...

Poltorak, V.A.; Pankratova, V.N.; Kulalaeva, Z.I., 1973: Luminescence microscopic study of the localization of flavomycoin in Actinomyces brunneofungus. Mikrobiologiia 42(4): 698-702

Tosolini, A.P.; Morris, R., 2012: Spatial characterization of the motor neuron columns supplying the rat forelimb. Rats can generate a rich array of forepaw and forelimb movements that are similar, although not as complex, to those produced by human and non-human primates. When reaching for food for instance, rats display skilled movements of the forelimb and...

Hillyard, D.R.; Monje, V.D.; Gaur, S.; Nadasdi, L.; Miljanich, G.; Ramachandran, J.; Oliver, B.M., 1992: Novel conopeptide inhibitors of mammalian presynaptic calcium channels derived from cDNA cloning and peptide synthesis. Society for Neuroscience Abstracts 18(1-2): 970

Zhang, L.; Lidow, M.S., 2001: Differential D1 dopamine receptor regulation of proliferation of EGF- and FGF2-supported cerebral cortical stem cells. Previously we demonstrated the capability of dopamine D1 receptor in regulating cell proliferation in EGF-supported mouse cerebral cortical stem cells in primary cultures. Recent findings indicated that the fetal cortical proliferative matrix cont...

Brown, A.W.A.; Armstrong, J.A.; Peterson, D.G., 1954: Investigation of suspected resistance of mosquitoes to DDT in southern Ontario. A total of 33 collections of mosquito larvae were taken in 1953 from various localities, some previously treated with DDT and others never treated, and their susceptibilities to DDT in both the larval and adult stages were compared in the laborato...

Robertson, B.E.; Kozlowski, R.Z.; Nye, P.C.G., 1992: Opposing actions of tolbutamide and glibenclamide on hypoxic pulmonary vasoconstriction. We show that cromakalim and diazoxide, drugs that activate ATP-sensitive potassium (K-ATP) channels, abolish hypoxic pulmonary vasoconstriction (HPV) of isolated, perfused rat lungs. Glibenclamide, an inhibitor of these channels, does not affect H...

MANOLOV.P.; DONEV.N.; DALEVA.L., 1964: Spasmolytic Function Of Some Phenylbutylamines. Suvremenna Meditsina 15: 10-16

Hoogerkamp, M., 1974: Experiences with less frequent mowing and spraying of roadside verges. In maintenance trials on roadside grass verges the effects on the grass and neighbouring vegetation of a reduction in herbicide sprays, were investigated together with less frequent mowing and the removal of grass mowings. A reduction in mowing fr...

Anonymous, 1982: Varieties of fodder root crops, 1982. Farmers leaflet National Institute of Agricultural Botany: (6)