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Characterization of deoxyribonucleic acid from cells infected with Aleutian disease virus


, : Characterization of deoxyribonucleic acid from cells infected with Aleutian disease virus. American Journal of Veterinary Research 44(7): 1177-1181

Viral DNA was extracted from Crandell feline kidney (CRFK) cells infected with Aleutian disease virus (ADV) and labelled with thymidine. The sedimentation coefficient in alkaline sucrose gradients was 16S corresponding to a molecular weight of 1.5 million. The buoyant densities of DNA from infected and control cells were determined by isopyknic sedimentation in CsCl and NaI gradients. Two additional peaks of DNA were found in infected cells, but not in control cell extracts.

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Hahn E.C.; Ramos L.; Kenyon A.J., 1983: Characterization of dna from cells infected with aleutian disease virus. Viral DNA was extracted from Crandell feline kidney (CRFK) cells infected with Aleutian disease virus (ADV) and labeled with [3H]thymidine. The sedimentation coefficient in alkaline sucrose gradients was 16S corresponding to a MW of 1.5 .times. 10...

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