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Characterization of in vitro proton pumping by microsomal vesicles isolated from corn coleoptiles

, : Characterization of in vitro proton pumping by microsomal vesicles isolated from corn coleoptiles. Plant Physiology 70(6): 1738-1742

Microsomal vesicles isolated from maize coleptiles had an Mg-ATP-dependent proton transport mechanism which was measured by quenching of quinacrine fluorescence and 14C-methylamine accumulation. The proton pump was temp.-sensitive, had a pH opt. of 7.5, was stimulated by Cl, insensitive to k and inhibited by carbonyl cyanide-m-chlorophenylhydrazone. Mn was almost as stimulatory as Mg2+ but high levels of Ca2+ were inhibitory.

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Accession: 001053403

PMID: 16662754

DOI: 10.1104/pp.70.6.1738

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