Complementation in somatic hybrids indicates four types of nitrate reductase deficient lines in Nicotiana plumbaginifolia

Marton, L.; Sidorov, V.; Biasini, G.; Maliga, P.

Molecular and General Genetics 187(1): 1-3

1982


DOI: 10.1007/bf00384375
Accession: 001057006

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Abstract
Allelism of nine nitrate reductase deficient (NR-) cell lines was tested by complementation after protoplast fusion. Complementation was recognized by the appearance of somatic hybrid colonies growing on a selective NH4+/NO3- medium which could not support the growth of NR- lines. Five apoenzyme-defective (NA) lines were noncomplementing and therefore allelic. Apoenzyme and cofactor-defective (NX) lines were complementing, as expected, and gave somatic hybrids with restored nitrate reductase activity. The four cofactor-defective lines were found to belong to three complementation groups (NX1 and NX9; NX21; NX24). NX21 and NX24 are new types which have not previously been described in flowering plants. In the somatic hybrids restoration of NR activity was accompanied by the restoration of plant regeneration ability.

Complementation in somatic hybrids indicates four types of nitrate reductase deficient lines in Nicotiana plumbaginifolia