+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Differentiation of infectious laryngotracheitis virus strains using restriction endonucleases



Differentiation of infectious laryngotracheitis virus strains using restriction endonucleases



Avian Diseases 26(4): 718-731



Strains of infectious laryngotracheitis virus (ILTV) were examined using an indirect immunofluorescent test (IIF) and with restriction endonucleases to detect intratypic differences. Electrophoretic analysis of ILTV DNA fragments cleaved with restriction endonuclease HindIII clearly distinguished between strains. The IIF test did not discriminate between strains. A MW estimate of ILTV DNA was made by summation of restriction endonuclease fragments cleaved with BamHI (102.1 .times. 106) and HindIII (97.35 .times. 106). Differences between the estimates may indicate the presence of submolar fragments.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 001063403

Download citation: RISBibTeXText

PMID: 6297444

DOI: 10.2307/1589858


Related references

Differentiation of vaccine strains and Georgia field isolates of infectious laryngotracheitis virus by their restriction endonuclease fragment patterns. Avian Diseases 34(3): 646-656, 1990

Rapid differentiation of vaccine strains and field isolates of infectious laryngotracheitis virus by restriction fragment length polymorphism of PCR products. Journal of Virological Methods 66(2): 179-186, 1997

Differences among restriction endonuclease DNA fingerprints of Pennsylvania field isolates, vaccine strains, and challenge strains of infectious laryngotracheitis virus. Avian Diseases 36(3): 575-581, 1992

Differentiation of isolates of infectious laryngotracheitis virus through restriction endonuclease analysis. Poultry Science 68(Suppl. 1): 64, 1989

Differentiation of infectious laryngotracheitis virus strains by polymerase chain reaction. Avian Diseases 41(1): 241-246, 1997

Analysis of Korean strains of infectious laryngotracheitis virus by nucleotide sequences and restriction fragment length polymorphism. Veterinary microbiology 83(4): 321-331, 2001

Characterization of field strains of infectious laryngotracheitis virus in China by restriction fragment length polymorphism and sequence analysis. Journal of Veterinary Diagnostic Investigation 28(1): 46-49, 2016

Differentiation of field isolates and vaccine strains of infectious laryngotracheitis virus by DNA sequencing. Vaccine 27(48): 6731-6738, 2009

Differentiation between virulent and avirulent strains of infectious laryngotracheitis virus by Dna: Dna hybridization using a cloned Dna marker. Veterinary Microbiology 11(4): 319-330, 1986

Differentiation between virulent and avirulent strains of infectious laryngotracheitis virus by DNA:DNA hybridization using a cloned DNA marker. Veterinary Microbiology 11(4): 319-330, 1986

Characterization by restriction fragment length polymorphism and sequence analysis of field and vaccine strains of infectious laryngotracheitis virus involved in severe outbreaks. Avian Pathology 39(6): 425-433, 2010

Restriction endonucleases recognizing DNA sequences of four base pairs facilitate differentiation of herpes simplex virus type 1 strains. Archives of Virology 97(3-4): 197-214, 1987

Laryngotracheitis gallid 1 herpesvirus infection in the chicken 4. latency establishment by wild and vaccine strains of infectious laryngotracheitis virus. Avian Pathology 15(3): 581-596, 1986

Characterization of western European field isolates and vaccine strains of avian infectious laryngotracheitis virus by restriction fragment length polymorphism and sequence analysis. Avian Diseases 52(2): 278-283, 2008

Differentiation of Infectious Laryngotracheitis Virus Isolates by Restriction Fragment Length Polymorphic Analysis of Polymerase Chain Reaction Products Amplified from Multiple Genes. Avian Diseases 50(1): 28-34, 2006