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Homologous terminal sequences of the genome double-stranded RNAs of bluetongue virus

Homologous terminal sequences of the genome double-stranded RNAs of bluetongue virus

Journal of Virology 46(2): 378-383

The 3'-terminal sequences of the 10 double-stranded RNA genome segments of bluetongue virus (serotypes 10 and 11) were determined. The double-stranded RNA were 3'-labeled with [5'-32P]pCp and resolved into 10 segments by electrophoresis. After denaturation, the 2 complementary strands of segments 4-10 were resolved into fast- and slow-migrating species by polyacrylamide gel electrophoresis, and their 3' end sequences were determined. Complete RNase T1 digestion of the individual 3'-labeled double-stranded RNA segments yielded 2 labeled oligonucleotides, one of which migrated faster than the other on 20% polyacrylamide-7 M urea gels. Sequence analyses of the 2 oligonucleotides of segments 4-10 confirmed the corresponding RNA sequence data. For RNA segments 1-3 the oligonucleotide analyses gave comparable results. The 3'-terminal sequences of the fast-migrating RNA sp. were HOCAAUUU ..; those of the slow-migrating RNA sp. were HOCAUUCACA.. Similar results were obtained for double-stranded RNA from bluetongue virus serotypes 10 and 11. Beyond the common termini, the sequences for each segment varied considerably.

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Accession: 001085429

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PMID: 6302309

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