+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Inhibitory effects of erythrocyte membrane proteins on the in vitro invasion of the human malarial parasite (Plasmodium falciparum) into its host cell



Inhibitory effects of erythrocyte membrane proteins on the in vitro invasion of the human malarial parasite (Plasmodium falciparum) into its host cell



Journal of Cell Biology 903: 563-567



The intracellular development of the erythrocytic stage of the malarial parasite (merozoite) is initiated by the attachment of the parasite to the erythrocyte surface. This paper describes an assay system to investigate Plasmodium falciparum merozoite entry into the host cell and reports on three observations regarding this interaction. (a) Merozoites do not invade human erythrocytes treated with either trypsin or neuraminidase, and both enzymes partially cleave glycophorin A, the major erythrocyte surface sialoglycoprotein. (b) A membrane protein fraction containing glycophorin A will, at low concentrations, inhibit the invasion of isolated merozoites into erythrocytes; no other fractions of membrane proteins have appreciable effects on the reinvasion. (c) Merozoites do not reinvade erythrocytes preincubated with F ab' fragments of antibody prepared against glycophorin A. Together, these three observations imply a role for glycophorin A in the attachment of the malarial parasite to the erythrocyte surface.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 001090858

Download citation: RISBibTeXText

PMID: 7026577

DOI: 10.1083/jcb.90.3.563


Related references

Inhibitory Effects of Erythrocyte Membrane Proteins on the in vitro Invasion of the Human Malarial Parasite (Plasmodium falciparum) into Its Host Cell. The Journal of Cell Biology 90(3): 563-567, 1981

Plasmodium falciparum proteins at the host erythrocyte membrane: their biological and immunological significance and novel parasite organelles which deliver them to the cell surface. The biology of parasitism A molecular and immunological approach: 111-145, 1988

Vesicle-mediated trafficking of parasite proteins to the host cell cytosol and erythrocyte surface membrane in Plasmodium falciparum infected erythrocytes. International Journal for Parasitology ober; 31(12): 1381-1391, 2001

The mechanism of erythrocyte invasion by the malarial parasite,Plasmodium falciparum. 2011

The mechanism of erythrocyte invasion by the malarial parasite, Plasmodium falciparum. Seminars in Cell and Developmental Biology 22(9): 953-960, 2011

Evidence for vesicle-mediated trafficking of parasite proteins to the host cell cytosol and erythrocyte surface membrane in Plasmodium falciparum infected erythrocytes. Molecular and Biochemical Parasitology 106(1): 131-145, 2000

Covalent modification of the permeability pathways induced in the human erythrocyte membrane by the malarial parasite Plasmodium falciparum. Journal of Cellular Physiology 133(1): 55-63, 1987

Plasmodium falciparum Plasmodium helical interspersed subtelomeric proteins contribute to cytoadherence and anchor P. falciparum erythrocyte membrane protein 1 to the host cell cytoskeleton. Cellular Microbiology 18(10): 1415-1428, 2016

Plasmodium falciparum proteins at the host erythrocyte membrane: their biological and immunological significance and novel parasite organelles which deliver them to the surface. MBL (Marine Biology Laboratory) Lectures in Biology 9: 111-145, 1988

Membrane specific mapping and colocalization of malarial and host skeletal proteins in the Plasmodium falciparum infected erythrocyte by dual-color near-field scanning optical microscopy. Proceedings of the National Academy of Sciences of the United States of America 94(2): 520-525, 1997

Metabolic interconnection between the human malarial parasite Plasmodium falciparum and its host erythrocyte. Regulation of ATP levels by means of an adenylate translocator and adenylate kinase. Journal of Biological Chemistry 264(6): 3194-3199, 1989

Effects of lysosomotropic detergents on the human malarial parasite Plasmodium falciparum in in vitro culture. Biochemical Pharmacology 38(8): 1271-1277, 1989

Bioflavonoid effects on in vitro cultures of Plasmodium falciparum. Inhibition of permeation pathways induced in the host cell membrane by the intraerythrocytic parasite. Biochemical Pharmacology 37(22): 4269-4276, 1988

Quantitative time-course profiling of parasite and host cell proteins in the human malaria parasite Plasmodium falciparum. Molecular and Cellular Proteomics 10(8): M110.006411, 2011

The effect of soluble glycophorin A on invasion of human red cells by the malarial parasite Plasmodium falciparum. Southeast Asian Journal of Tropical Medicine and Public Health 13(4): 662-663, 1982