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Production in the laboratory of apothecia of Sclerotinia trifoliorum Eriks. for the evaluation of resistance of red clover to Sclerotinia disease



Production in the laboratory of apothecia of Sclerotinia trifoliorum Eriks. for the evaluation of resistance of red clover to Sclerotinia disease



Agronomie 3(4): 369-373



Sclerotia were produced on 1-month-old cultures, desiccated for 1 month at room temp. and frozen for 24 h at -18 deg C. When covered with moist vermiculite they produced apothecia in 1 month at 15 deg under fluorescent light (18 W/m2) with a light period of 12 h/24. Apothecial production continued for at least 2 months, at the rate of 2000-3000/10 000 sclerotia, each apothecium yielding on av. 30 000 ascospores.

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