Activity and isoenzyme spectrum of lactate dehydrogenase during ascaridiasis in chicks

Selikhova, O.V.

Byulleten' Vsesoyuznogo Instituta Gel' mintologii im KI Skryabina 34: 48-51

1983


Accession: 001157951

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Abstract
Chicks of 2 lines (V-77 and V-66) were infected, when 30 days old, with 300 Ascaridia galli ova each and were killed 1 h, and at intervals until day 45 after infection. Lactate dehydrogenase (LDG) activity increased from day 3, to 25.4 micro moles/ml in V-77 and 24.7 micro moles/ml in V-66 chicks, and peaked on day 7, exceeding control levels by 12.3 and 8.1% respectively. The isoenzyme spectrum of LDH showed 2 molecular forms with 4 fractions: electrophoretically rapid LDG-1 and 2, an intermediate fraction - LDG-3 and a slow one - LDG-4. The activities of LDG-1 and 2 increased from day 3 to day 7, whilst the activity of LDG-4. The activities of LDG-1 and 2 increased from day 3 to day 7, whilst the activity of LDG-4 declined, to rise only as the intestinal inflammation subsided. A 2nd peak of activity of LDG-1 and 2 occurred as the larvae entered the intestine on day 21, when the total activity of LDG was at control level. A rise in LDG-4 activity and normalization of the other isoforms occurred on day 28. The level of enzymatic activity was higher in the V-77 than in the V-66 line.