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Adaptation of an immunosorbent technique (ELISA) for the detection of maize rayado fino virus in its insect vector






Turrialba 33(2): 177-183

Adaptation of an immunosorbent technique (ELISA) for the detection of maize rayado fino virus in its insect vector

An enzyme-linked immunosorbent assay (ELISA) was adapted in Costa Rica to detect maize rayado fino virus (RFV), which is widely distributed in the New World tropics, in its vector Dalbulus maidis (DeLong & Wolc.). The best results were obtained when the experimental procedure included the use of anti-RFV immunoglobulin G (IgG) purified by ion exchange chromatography, the determination of critical concentrations of the enzyme-IgG conjugate, and the use of Tween 20 and bovine serum albumen.


Accession: 001158137



Related references

Rivera, C.; Gamez, R., 1983: Adaptation de una tecnica de immunosorbenica ELISA a la deteccion del virus del virus del rayado fino del maiz en su insecto vector. Turrialba, 336: 177-184

Gamez, R.; Leon, P., 1983: Maize rayado fino virus: evolution with plant host and insect vector. The biology of maize rayado fino virus (RFV) and its vectors (species of the euscelid genus Dalbulus, especially D. maidis) in America is discussed from an evolutionary and ecological point of view, and evidence is presented that supports the hypo...

Paniagua, R.G.mez, R., 1976: Maize rayado fino virus: additional studies on the relationship of the virus its insect vector. Turrialba: 26 (1) 39-43

Paniagua, R.; Gamez, R., 1976: Rayado fino virus of maize: additional studies on the relation between the virus and its insect vector. In further studies in Costa Rica on the relation between the rayado fino virus of maize and its vector Dalbulus maidis (DeLong & Wolc.) [see RAE/A 63, 5110, etc], the leafhopper was able to acquire the virus after an acquisition feed lasting 6 h a...

Rivera, C.; Gámez, R., 1986: Multiplication of maize rayado fino virus in the leafhopper vector Dalbulus maidis. The enzyme-linked immunosorbent assay (ELISA) was used to demonstrate the increase in titer of maize rayado fino virus (MRFV) in its leafhopper vector, Dalbulus maidis. Viral antigen concentration attained a maximum in the body of the insect 25 da...

Kitajima, E.W.; Gámez, R., 1983: Electron microscopy of maize rayado fino virus in the internal organs of its leafhopper vector. Discrete groups of 20- to 25-nm isometric particles, considered to represent maize rayado fino virus (RFV), were consistently observed in the ectoderm, fat body, midgut, follicular cells of the ovary, muscle, and salivary glands of RFV-infected Da...

Gamez, R., 1983: Maize rayado fino disease: the virus-host-vector interaction in Neotropical environments. Proceedings: International Maize Virus Disease Colloquium and Workshop Wooster Ohio August 2-6-1982 edited by DT Gordon et al: 8

Kitajima E.W.; Gamez R., 1983: Electron microscopy of maize rayado fino virus in the internal organs of its leafhopper dalbulus maidis vector. Discrete groups of 20-25 nm isometric particles, considered to represent maize rayado fino virus (RFV), were consistently observed in the ectoderm, fat body, midgut, follicular cells of the ovary, muscle and salivary glands of RFV-infected D. maid...

Rivera, C.; Kozuka, Y.; Gamez, R., 1981: Rayado fino virus: detection in salivary glands and evidence of increase in virus titer in the leafhopper vector Dalbulus maidis. The rayado fino virus of maize was detected in salivary glands of the vector Dalbulus maidis (DeLong & Wolc.) examined under the electron microscope. By means of the enzyme-linked immunoabsorbent assay, a gradual increase in the amount of virus in...

Ramirez P.; Karkashian J.; D.L.s Angeles Mora M.; Hammond R., 1990: Dot hybridization assay for detection of maize rayado fino virus mrfv in plant extract using biotinylated probes. Phytopathology 80(5): 516