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Adaptation of the Hoffmann and Teicher's method for the microdetermination of urease activity in soils






Anales de Edafologia y Agrobiologia 41(3-4): 617-626

Adaptation of the Hoffmann and Teicher's method for the microdetermination of urease activity in soils

Various aspects of the method were studied, including sample size, sample/reagents ratio, containers, shaking and form of heat applied.


Accession: 001158153



Related references

Gonzalez-Carcedo, S.B.rriuso-Benito, E., 1982: Problems arosen in the adaptation of the Hoffmann and Teichers method for urease activity microtrace determination in soils. Anales de edafologia y agrobiologia 41(3-4): 617-626

Dixon, N.; Blakeley, R.; Zerner, B., 1980: Jackbean urease (EC 3.5.1.5). I. A simple dry ashing procedure for the microdetermination of trace metals in proteins. The nickel content of urease. Canadian journal of biochemistry = Journal canadien de biochimie 58(6): 469-473

Dixon, N.E.; Blakeley, R.L.; Zerner, B., 1980: Jack bean urease (EC 3.5.1.5). I. A simple dry ashing procedure for the microdetermination of trace metals in proteins. The nickel content of urease. A simple and inexpensive procedure for determination of microgram quantities of metal ions in proteins is described and tested with nickel and iron. The method involves (a) dry ashing in an oxygen atmosphere at 450-460 degrees C in Pyrex vessels,...

Douglas, L.A.; Bremner, J.M., 1971: A rapid method of evaluating different compounds as inhibitors of urease activity in soils. A simple method of studying the abilities of different compounds to inhibit urease activity in soils is described. It involves determination of the effect of the test compound on the amount of urea hydrolysed by incubation of soils with urea and t...

Kozlovskaia, N.; Nikitina, G.; Runkov, S., 1972: Microdiffusion express method for determining urease activity of peat-bog soils. Agrokhimiia 5 144-149

Kozlovskaya, N.A.; Nikitina, G.D.; Runkov, S.V., 1972: A rapid micro-diffusion method for the determination of the urease activity of peat-bog soils. Peat is incubated with urea in a thermostat for 1-2 hr. NH4-N is determined by a micro-diffusion method with Nessler's reagent.

Praveen Kumar P.K.C.; Burman U., 1990: Effect of urease inhibitors on energy barriers of urease activity of soils. A linear Arrhenius plot was obtained in acidic soil whereas a non-linear plot with the inflexion at 30.degree. C (critical temperature) was observed in alkaline and saline soils which can be attributed to the sudden change in conformation of enzym...

Guettes, R.; Dott, W.; Eisentraeger, A., 2002: Determination of urease activity in soils by carbon dioxide release for ecotoxicological evaluation of contaminated soils. A method for the quantification of urease enzyme activity was set up, which is based on the quantification of carbon dioxide set free into the head space of gastight vessels. The method can be applied for ecotoxicological characterisation of conta...

Pfanneberg, T.F.scher, W.; Niederbudde, E., 1978: Determination of urease activity by two different methods in some soils and underwater soils

Zhao Lue; Sun QingYuan; Y.Y.ngMei; M.G.oBao, 2007: Effect of urease inhibitor nBPT on soil urease activity and urease producing microorganisms. The cell growth and the releasing rate of urease from bacteria and actinomycete in the presence of different n-butyl thiophosphoric triamide (nBPT) concentrations were examined. The results showed that the effect of nBPT on soil urease activity wa...