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Aggregation characteristics of carnation ringspot virus in vitro



Aggregation characteristics of carnation ringspot virus in vitro



Acta Phytopathologica Academiae Scientiarum Hungaricae 18(1/3): 85-99



The influence of temp., medium composition and virus concn on reversible aggregation was investigated by measuring the transmission of CRSV solutions at lambda = 400 nm. In 0.01 M phosphate buffer, pH 5, with 5 mg/ml CRSV, aggregation could be observed at 35 degrees C, with 0.15 mg/ml only at 71 degrees. At low virus concn aggregation was favoured by 1 M NaCl or KCl or 0.1 M MgCl2, and was inhibited by 20% w/v sucrose.

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Accession: 001158788

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