Section 2
Chapter 1,161

An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Nairobi sheep disease virus in comparison with an indirect immunofluorescent and hemagglutination test. I. Development and testing of the ELISA with sera of experimentally infected rabbits and sheep

Munz, E.; Reimann, M.; Meier, K.

Zentralblatt für Veterinarmedizin. Reihe B. Journal of Veterinary Medicine. Series B 30(7): 473-479


ISSN/ISBN: 0514-7166
PMID: 6650018
DOI: 10.1111/j.1439-0450.1983.tb01870.x
Accession: 001160864

An ELISA for detecting IgG antibodies to Nairobi sheep disease bunyavirus employed part purified tissue culture antigen derived from infected and sonicated BHK cells. Rabbits were infected 2-3 times and high antibody titres of up to 1:5120 were detected for 10 months. Two of 3 infected sheep showed clinical symptoms 2-3 days after infection, with high fever accompanied by bloody and severe diarrhoea and anorexia for 9-11 days. The other sheep showed only high fever and apathy. Titres of 1:20 were detected for the first time 14 days after infection, increasing to 1:160 at 33 days. The test was highly sensitive, simple to perform, and suitable for large-scale epidemiological surveys.

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