Characterization of intraspecific somatic hybrids of carrot obtained by fusion of iodoacetate-inactivated A2CA-resistant and sensitive protoplasts

Cella, R.; Carbonera, D.; Iadarola, P.

Zeitschrift fur Pflanzenphysiologie 112(5): 449-457


DOI: 10.1016/s0044-328x(83)80151-2
Accession: 001171053

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The appearance of amino acid analogue A2CA (azetidine-2-carboxylic acid) resistant colonies in a mixed population of iodoacetate-inactivated (unable to regenerate cell walls and develop colonies) A2CA-resistant and sensitive carrot protoplasts depended upon fusion. No colonies arose from controls in which a mixed unfused (or homologously fused) population of parental protoplasts were plated. Suspension cultures obtained from resistant colonies were tested for level of resistance to A2CA, uptake of the analogue and endocellular content of free proline. The values for these characters were intermediate between those of the parental lines. The chromosome number of hybrid cell lines was predominantly aneuploid, but ranged from 2n = 18 to 67 in a single line. The most common chromosome number in the hybrid cell lines was similar to that of the sensitive parent, and was in the range 2n = 19-35, suggesting that extensive chromosome loss had occurred in the fusion products.