Continuous hydrolysis of lactose in skim milk and acid whey by immobilized lactase of Aspergillus oryzae
Hirohara, H.; Yamamoto, H.; Kawano, E.; Nagase, T.
Enzyme Engineering 6: 295-297
1982
Accession: 001176204
Purified beta -D-galactosidase from Aspergillus oryzae was covalently immobilized onto a phenolic amphoteric ion-exchange resin using glutaraldehyde. In a continuous operation, a space velocity of nearly 60/h gave 80% hydrolysed whey at 45-50 degrees C. Higher productivities were obtained at lower temp. because of long half-lives, although reaction rates were slow.
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