+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

DNA polymorphisms and subpopulations in Babesia bovis



DNA polymorphisms and subpopulations in Babesia bovis



Molecular and biochemical parasitology 11(11): 91-103



Independent isolates of Babesia bovis differ by only a limited number of polypeptides, some of which may be important as host protective antigens. Avirulent derivatives of these parasites also differ from their virulent counterparts in only a few polypeptides. To identify genes encoding such polypeptides we have isolated cDNA clones corresponding to poly(A)+ RNAs that are expressed only in certain isolates. For this purpose a cDNA clone library was constructed from poly(A)+ RNA of the K-avirulent isolate (KA). These clones were screened by colony hybridization using [32P]cDNA complementary to poly(A)+ RNA from KA and from virulent isolates, in order to identify clones that selectively hybridize to one cDNA probe. Hybridization of DNA from three clones, designated pK4, pK5 and pK6 to poly(A)+ RNA from various isolates revealed different and complex patterns. The gene represented by clone pK5 appeared to be transcribed predominantly in avirulent parasites. Analysis of genomic DNA by the Southern procedure enabled each isolate to be distinguished and suggested that most isolates are comprised of a heterogeneous mixture of subpopulations. Analysis of genomic DNA from parasites obtained after passage of KA through the tick vector (Boophilus microplus) suggested that a subpopulation was being selected that more closely resembled KV than KA.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 001178973

Download citation: RISBibTeXText

PMID: 6205269

DOI: 10.1016/0166-6851(84)90057-4


Related references

Babesia bovis transovarian transmission in Boophilus microplus obtention of a Babesia free tick strain Transmissao transovariana de Babesia bovis em Boophilus microplus obtencao de cepa de carrapato livre de Babesia spp. Ciencia Rural. mai-jun; 303: 455-459, 2000

Babesia bovis, Babesia bigemina, Babesia canis, Babesia microti and Babesia rodhaini: comparison of ribosomal RNA gene organization. International Journal for Parasitology 22(6): 851-855, 1992

Babesia bovis: evidence for selection of subpopulations during attenuation. Experimental Parasitology 704: 404-410, 1990

Ixodes ricinus experimental vector of 3 species of babesia babesia bovis babesia berbera and babesia argentina. Revue Suisse de Zoologie 83(2): 443-462, 1976

Ixodes ricinus, an experimental vector of three species of -Babesia (Babesia bovis, Babesia berbera, Babesia argentina). Revue Suisse de Zoologie 3(2): 443-462, 1976

The detection of large merozoites vermicules of babesia in boophilus microplus females engorged on calves with different levels of parasitemia of babesia bigemina and babesia bovis babesia argentina. Revista Iberica de Parasitologia 45(4): 303-312, 1985

Oviposition in Boophilus microplus infected artificially with Babesia bovis and naturally with B. bovis and Babesia bigemina. Annales de Parasitologie Humaine et Comparee 68(4): 196-198, 1993

Deteccion de merozoitos grandes (vermiculos) de Babesia en teleoginas de Boophilus microplus alimentadas sobre terneros con distintos niveles de parasitemia de Babesia bigemina y Babesia bovis (=Babesia argentina). Revista Iberica de Parasitologia 454: 303-311, 1985

Analysis of the composition of samples of Babesia bovis and the influence of different environmental conditions on genetically distinct subpopulations. International Journal For Parasitology. 22(6): 731-737, 1992

An Evaluation of Quantitative PCR Assays (TaqMan ® and SYBR Green) for the Detection of Babesia bigemina and Babesia bovis, and a Novel Fluorescent-ITS1-PCR Capillary Electrophoresis Method for Genotyping B. bovis Isolates. Veterinary Sciences 3(3):, 2016

In vitro culture of Babesia bovis under different management conditions Cultivo in vitro de Babesia bovis bajo diferentes condiciones de manejo. Veterinaria Tropical. Julio-Diciembre; 262: 89-108, 2001

Characterisation of a family of multi-copy genes encoding rhoptry protein homologues in Babesia bovis, Babesia ovis and Babesia canis. Molecular and Biochemical Parasitology 57(2): 181-192, 1993

Babesia rodhaini, Babesia bovis, and Babesia bigemina: analysis and sorting of red cells from infected mouse or calf blood by flow fluorimetry using 33258 Hoechst. Experimental Parasitology 48(3): 421-431, 1979

Estudios inmunoserologicos comparativos entre cepas de Anaplasma marginale, Theiler, 1910; Babesia bigemina, Smith y Kilborne, 1893; Babesia bovis, Babes, 1888 y Babesia argentina, Lignieres, 1903. Revista Cubana de Ciencias Veterinarias 152: 117-123, 1984

Genetic characterization and molecular survey of Babesia bovis, Babesia bigemina and Babesia ovata in cattle, dairy cattle and yaks in China. Parasites and Vectors 8: 518, 2015