EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Detection of potato spindle tuber viroid by nucleic acid spot hybridization: evaluation with tuber sprouts and true potato seed


Detection of potato spindle tuber viroid by nucleic acid spot hybridization: evaluation with tuber sprouts and true potato seed



American Potato Journal 60(8): 587-597



ISSN/ISBN: 0003-0589

DOI: 10.1007/bf02854108

A diagnostic test based on hybridization of highly radioactive, recombinant DNA complementary to PSTV with PSTV bound to a nitrocellulose membrane and autoradiographic detection of the resulting DNA-RNA hybrids was evaluated with tubers from 20 clones maintained at the CIP and with true seed obtained from healthy or infected plants.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 001180373

Download citation: RISBibTeXText

Related references

Comparative detection of mild strains of potato spindle tuber viroid from the dormant potato tubers by return-polyacrylamide gel electrophoresis and nucleic acid hybridization. Potato Research 31(1): 159-166, 1988

Comparison of four radioactive probes for the diagnosis of potato spindle tuber viroid by nucleic acid spot hybridization. Potato Research 31(3): 431-442, 1988

Detection of potato spindle tuber viroid in true potato seed. Phytopathology 72(7): 962, 1982

Detection of potato spindle tuber viroid in true potato seed by bioassay on rutgers tomato. American Potato Journal 64(5): 235-244, 1987

Chemiluminescent detection of potato spindle tuber viroid in true potato seed using a digoxigenin labelled DNA probe. Potato Research 37(3): 249-255, 1994

Detection of the mild strains of potato spindle tuber viroid from single true potato seed by return electrophoresis. Phytopathology 78(6): 663-667, 1988

Detection distribution and long term persistence of potato spindle tuber viroid in true potato seed from heilongjiang china. American Potato Journal 68(1): 65-74, 1991

Determination of analytic parameters of nucleic acid hybridization techniques for potato spindle tuber viroid (PSTVd) detection. Fitopatologia 36(1): 24-33, 2001

Effect of potato spindle tuber viroid on sexual reproduction and viroid transmission in true potato solanum tuberosum seed. Canadian Journal of Botany 64(2): 336-340, 1986

Effect of potato spindle tuber viroid on sexual reproduction and viroid transmission in true potato seed. Canadian Journal of Botany 64(2): 336-340, 1986

Potato spindle tuber viroid rna oligomers of dextro and levo polarity are synthesized in potato protoplasts after liposome mediated infection with potato spindle tuber viroid. Virus Research 4(3): 213-228, 1986

Sensitive and rapid diagnosis of potato spindle tuber viroid disease by nucleic Acid hybridization. Science 213(4508): 670-672, 1981

Oligomers of potato spindle tuber viroid are synthesized in potato protoplasts after liposome mediated infection with potato spindle tuber viroid. Biological Chemistry Hoppe-Seyler 366(9): 785-786, 1985

Detection of potato spindle tuber viroid (PSTVd) in minute amounts of potato (Solanum tuberosum L.) leaf tissue by hybridization techniques and, together with potato viruses, by multiplex RT-PCR. Journal of Plant Diseases and Protection 116(3): 97-105, 2009

Erfahrungen zum Routinenachweis des potato spindle tuber viroid - Expérience acquise de la détection en routine du viroïde du tubercule en fuseau de la pomme de terre - Experience from routine detection of potato spindle tuber viroid. Archiv für Phytopathologie und Pflanzenschutz 26(4): 405-413, 1990