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Detection of potato viruses with an ultra-micro variant of enzyme-linked immunosorbent assay (ELISA)

Reichenbacher, D.; Kalinina, I.; Kleinhempel, H.; Schulze, M.; Horn, A.

Archiv fur Phytopathologie und Pflanzenschutz 20(2): 185-187

1984


Accession: 001180376

Using the method of Horn et al. (Journal of Clinical Chemistry and Clinical Biochemistry (1981) 19, 702-706) potato viruses X, Y and M in secondarily infected potato plants and potato leaf roll virus in primarily infected plants were detected with the same reliability as by the usual micro-standard ELISA, but with a reduction of 90-95% in the quantities of chemicals, antibodies and alkaline phosphatase required.

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