Estimating progesterone in samples of bovine blood and milk by means of different radio-immunoassay and enzyme-immunoassay methods

Hoedemaker, M.; Arnstadt, K.I.; Grunert, E.

Zentralblatt fur Veterinarmedizin, A 31(2): 105-118


Accession: 001195677

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Estimates of progesterone in 232 samples of milk fat from 39 cows by radio-immunoassay (RIA) as developed in the thesis by the 1st author [DSA 45, 5184] were usually lower than corresponding estimates by enzyme-immunoassay (EIA) using the method of Arnstadt & Cleere [Journal of Reproduction and Fertility (1981) 62 (1) 173-180, DSA 43, 6077], mean values being 169 +or- 77 and 207 +or- 81 ng/ml, resp. The correlation between estimates by the 2 methods was 0.7 (P 0.001). The intra- and inter-assay coeff. of variation (C.V.) were 8.4 and 9.7% for RIA and 7.7 and 11.9% for EIA. Direct EIA of progesterone in whole milk using the method of Arnstadt & Schmidt-Adamopoulou [British Veterinary Journal (1982) 138, 436-438, DSA 44, 8945] gave a mean of 19.4 +or- 10 ng/ml (152 samples) and had intra- and inter-assay C.V. of 11.3 and 14.2%. Correlation coeff. for RIA progesterone concn. in blood plasma with RIA and EIA progesterone in milk fat and EIA progesterone in milk were 0.02, 0.17 and 0.30, resp.