+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Evaluation of ELISA and electron microscopy for the detection of coronavirus and rotavirus in bovine faeces

Evaluation of ELISA and electron microscopy for the detection of coronavirus and rotavirus in bovine faeces

Veterinary Record 114(16): 397-401

Enzyme linked immunosorbent assay (ELISA) was compared with electron microscopy in the examination of faeces from experimental calves and showed 100% agreement in the detection of 19 bovine coronavirus and 15 bovine rotavirus electron microscope positive samples. In a limited field survey of calf diarrhoea, 75 selected faecal samples were examined independently by ELISA and electron microscopy and the agreement between the two tests was 95% for coronavirus and 84% for rotavirus.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 001196288

Download citation: RISBibTeXText

PMID: 6328730

Related references

Comparison of the ELISA and immune electron microscopy for detection of rotavirus and coronavirus in calf faeces. Atti della Societa Italiana di Buiatria 24: 521-526, 1992

Solid phase immuno-electron microscopy (SPIEM) for rapid detection of rotavirus and coronavirus in faeces of calves, in comparison with conventional negative contrast. "Solid Phase" Immunelektronenmikroskopie SPIEM zum Schnellnachwies von Rota und Coronavirus in Kalberkotproben im Vergleich zum ublichen Negativ Kontrastverfahren: 129pp., 1989

Comparison of electron microscopy, enzyme-linked immunosorbent assay and latex agglutination for the detection of bovine rotavirus in faeces. Journal of the South African Veterinary Association 68(3): 93-96, 1997

ELISA for detection of bovine coronavirus in faeces and intestinal contents. Current topics in veterinary medicine and animal science2(22): 161-169, 1982

Detection of bovine coronavirus antigen in calf faeces by ELISA using monoclonal antibody. Chinese Journal of Veterinary Medicine 21(1): 8-11, 1995

Pseudoreplica electron microscopy for the detection of rotavirus: comparison with high-speed centrifugation electron microscopy and ELISA. Journal of Virological Methods 9(1): 79-85, 1984

Solid phase immunoelectron microscopy (SPIEM) for the quick detection of rotavirus and coronavirus in calf stool samples in comparison to usual electron microscopy methods. 1989

Detection by electron microscopy of caliciviruses, astroviruses and rotavirus-like particles in the faeces of piglets with diarrhoea. Veterinary Record 107(23): 532-533, 1980

Detection of rotavirus and corona like virus in stool samples of infected pigs by solid phase immune electron microscopy comparison of solid phase immune electron microscopy with direct electron microscopy and enzyme linked immunosorbent assay elisa. Ultramicroscopy 15(4): 396, 1984

Development of monoclonal antibody ELISA for simultaneous detection of bovine coronavirus, rotavirus serogroup A, and Escherichia coli K99 antigen in feces of calves. American Journal of Veterinary Research 53(1): 36-43, 1992

Comparing electron microscopy and a competitive blocking ELISA in the detection of rotaviruses in porcine faeces. Veterinary Journal 187(2): 279-281, 2011

Standardisation of an improved ELISA for the detection of rotavirus in faeces and its comparison with a commercial ELISA. Medical Science Research 25(11): 731-734, 1997

Detection of human rotavirus by reversed passive hemagglutination (RPHA) using antibody against a cultivable human rotavirus as compared with electron microscopy (EM) and enzyme-linked immunosorbent assay (ELISA). Microbiology and Immunology 26(8): 747-751, 1982

Comparison of ELISA with electron microscopy, and PAGE for the detection of human rotavirus in stools. Journal of the Medical Association of Thailand 71(8): 436-442, 1988

Incidence of diarrhoea and of rotavirus- and coronavirus-shedding in calves, whose dams had been vaccinated with an experimental oil-adjuvanted vaccine containing bovine rotavirus and bovine coronavirus. Journal of Veterinary Medicine, B Infectious Diseases, Immunology, Food Hygiene, Veterinary Public Health 35(3): 186-196, 1988