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In vitro and in vivo inactivation of penicillin by penicillinase from Staphylococcus aureus of bovine mammary origin

Kerala Journal of Veterinary Science 12(1): 31-35

In vitro and in vivo inactivation of penicillin by penicillinase from Staphylococcus aureus of bovine mammary origin

A penicillinase-producing strain of Staphylococcus aureus was isolated from normal bovine udder. The penicillinase hydrolysed benzyl penicillin, penicillin V and ampicillin during incubation at 37 degrees C for 60 min, but had no effect on cephalosporin or cloxacillin. A crude enzyme preparation inhibited the effects of penicillin in vitro, and inactivation of penicillin was also demonstrated in vivo using mice challenged with Bacillus anthracis.

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Accession: 001210669

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