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Inducement of cytopathic changes and plaque formation by porcine haemagglutinating encephalomyelitis virus



Inducement of cytopathic changes and plaque formation by porcine haemagglutinating encephalomyelitis virus



Veterinary Microbiology 8(6): 521-530



ESK [swine embryo kidney] cells were shown to be a good medium for propagating the 67N strain of porcine hemagglutinating encephalomyelitis virus, although no cytopathic effect was observed. The virus induced a readily recognizable cytopathic effect in ESK cells, when a non-cytotoxic amount of diethylaminoethyl-dextran (DEAE-dextran) was incorporated in the culture medium. Based on this finding, a sensitive, practical assay method for the virus was developed. When DEAE-dextran was incorporated in the agar overlay medium, 67N virus formed plaques in ESK cell monolayers. The cytopathic effect and the plaque formation were specifically inhibited by antisera against the virue. Neutralization tests were develped on the basis of these findings. Neutralization and hemagglutination-inhibition tests on swine serum samples indicated a wide dissemination of hemagglutinating encephalomyelitis virus or antigenically-related viruses in Japanese pigs.

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Accession: 001211667

Download citation: RISBibTeXText

PMID: 6659311

DOI: 10.1016/0378-1135(83)90001-9


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