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Induction of humoral neutralising and haemagglutination-inhibiting antibody by the spike protein of avian infectious bronchitis virus



Induction of humoral neutralising and haemagglutination-inhibiting antibody by the spike protein of avian infectious bronchitis virus



Avian Pathology 13(3): 573-583



The spike (S), membrane (M) and nucleocapsid (N) proteins of avian infectious bronchitis virus strain M41 (IBV-M41) were separated by sucrose gradient sedimentation after dissociation of the virus by nonionic detergent. Groups of 4 chickens were inoculated i.m. with 20 .mu.g of S, M or N in Freund's complete adjuvant and at 4 and 7 wk later with 20 .mu.g of S, M or N protein in Freund's incomplete adjuvant. Chickens were bled at 4, 7, 10 and 13 wk after the 1st vaccination and the sera analyzed for serum virus neutralizing (SN) and hemagglutination-inhibiting (HAI) antibody. Sera from all 4 chickens inoculated with S contained SN and HAI antibody. Maximum titers, in the range 5 to 9 log2, were attained after 1, 2 or 3 injections in 1, 2 and 1 chickens, respectively. The SN and HAI titers rose in parallel. Neither M nor N induced detectable SN or HAI antibody. None of the chickens resisted challenge to the respiratory tract at 6 wk after the final vaccination. Sera from chickens which had been infected twice with live IBV-M41 immunoprecipitated more radiolabeled S than N protein and little or no M. Similar results were obtained with sera from guinea pigs which had been inoculated i.m. with inactivated virus.

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Accession: 001211741

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PMID: 18766869

DOI: 10.1080/03079458408418556


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