+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The effect of protein quality and fibre level in the diet and microbial activity in the digestive tract on protein utilization and energy digestibility in rats



The effect of protein quality and fibre level in the diet and microbial activity in the digestive tract on protein utilization and energy digestibility in rats



British Journal of Nutrition 51(2): 305-314



Two N-balance experiments were performed with growing rats to test the effect of dietary fiber level, protein quality and antibiotic inclusion on microbial activity, N excretion patterns and energy digestibility. Each experiment involved 8 dietary treatments in a 2 x 2 x 2 factorial design, with 5 rats per treatment. The 8 treatments resulted from a combination of 2 protein treatments, 2 fiber treatments and 2 antibiotic treatments. In experiment 1, the protein was provided as barley, or barley plus 2 g L-lysine hydrochloride/kg dry matter (DM) (at 15 g N/kg DM) and in experiment 2 as soy bean meal or soy bean meal plus 2 g DL-methionine/kg DM (at 15 g N/kg DM). In both experiments the basal diet was provided with or without fiber as 100 g barley husk/mg DM and with of without antibiotic as 7 g Nebacitin/kg DM. With both barley and soy bean meal, true protein digestibility (TD) was improved with the addition of amino acids. Only with the soy bean meal diets was TD increased with Nebacitin treatment, with the effect of Nebacitin and methionine being additive. Barley husk slightly reduced the TD of soy bean meal. The effect of treatments on biological value (BV) was considerable. Lysine increased BV of the barley diet from 0.771 to 0.815 whereas Nebacitin reduced BV from 0.799 to 0.757. Methionine increased the BV of soy bean meal from 0.754 to 0.911 while BV was reduced by Nebacitin from 0.843 to 0.821 and by barley husk from 0.845 to 0.820. Net protein utilization (NPU) was markedly improved by the addition of amino acids and reduced by the addition of Nebacitin. Barley husk reduced NPU with soy bean meal diets only. Amino acid addition had no effect on energy digestibility of either diet, but Nebacitin reduced this value by .apprx. 5% on the barley diets, with a smaller reduction on the soy bean meal diets. Fiber reduced energy digestibility of both diets. On both diets, blood urea (BU) decreased with addition of amino acids. Nebacitin increased BU with the barley diets, but decreased BU with the soy bean meal diets. With the soy meal diets, the decrease with a combination of barley husk and methionine was particularly marked. Microbial activity, as measured by cecal ATP activity, was strongly reduced by antibiotic addition with both protein sources. On the soy bean meal diets ATP activity was increased by the addition of methionine. The results demonstrated a difference in response to the addition of fiber and the first limiting amino acid to barley and soy bean meal, as measured by several indicators of protein and energy utilization.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 001265560

Download citation: RISBibTeXText

PMID: 6322837

DOI: 10.1079/bjn19840035


Related references

The influence of dietary crude fibre and microbial activity in the digestive tract on true protein digestibility and biological value in rats. Zeitschrift fur Tierphysiologie, Tierernahrung und Futtermittelkunde 49(4/5): 173-180, 1983

The effect of provision of the first-limiting amino acid, gastrointestinal microbial activity and the level of nitrogen intake on protein utilization and energy digestibility in rats. British Journal of Nutrition 54(3): 727-739, 1985

Influence of diet and microbial activity in the digestive tract on digestibility, and nitrogen and energy metabolism in rats and pigs. British Journal of Nutrition 48(1): 161-175, 1982

The effect of dietary fibre level and microbial activity in the digestive tract on fat metabolism in rats and pigs. Acta Agriculturae Scandinavica 32(2): 145-150, 1982

Influence of the quality of diet protein on hydrolases of the digestive tract. 6. Effect of the amino acid pattern of the diet protein on autolytic capacity of the small intestine mucosa of rats. Nahrung. 15: 6, 653-662, 1971

Effect of environmental temperature on digestive tract, visceral organ size, digestibility and energy metabolism in rats fed different levels of pea fibre. Acta Agriculturae Scandinavica Section A Animal Science 46(3): 183-192, 1996

Influence of the quality of diet protein on hydrolases of the digestive tract. 4. Dependence of the proteolytic activity of the small intestine mucosa on the biological value of the diet protein. Nahrung. 15: 4, 353-362, 1971

Effect of the level of protein on digestibility of the diet and on energy balance in rats intoxicated with the fungicide Cynkotox. Bromatologia i Chemia Toksykologiczna 7(4): 453-457, 1974

Studies on the utilization of zinc from model diets by experimental rats. 7. Effect of a diet with an optimum level of Zn and different quality of protein on the levels of protein and nucleic acids in the liver of experimental rats. Zywienie Czowieka i Metabolizm 17(3): 171-180, 1990

Protein replacement by digestible fibre in the diet of growing rabbits. 1: Impact on digestive balance, nitrogen excretion and microbial activity. Animal Feed Science and Technology 183(3-4): 132-141, 2013

Effect of dietary fibre on protein and energy digestibility in rats. Revista Chilena de Nutricion 22(2): 115-119, 1994

The effect of varying crude protein level and the proportions of fibre in diets containing a mixture of meat-and-bone meal and wheat bran as the only protein source on nitrogen balance indices and energy digestibility in the rat. British Journal of Nutrition 56(2): 429-438, 1986

Digestibility of protein and absorption of amino acids in different sections of the digestive tract in pigs. 2. Protein and amino acid balances at the end of the small intestine and of the whole digestive tract (apparent and true protein and amino acid digestibility). Archiv fur Tierernahrung 29(4): 221-234, 1979

Protein digestibility and absorption of amino acids in various segments of the digestive tract of pigs. 2. Protein and amino acid balances at the end of the small intestine and of the whole digestive tract (apparent and true protein and amino acid digestibility). Archiv für Tierernahrung 29(4): 221-234, 1979

Effect of graded protein supply at a high energy level on fattening performance and retention and utilization of feed energy, protein and amino acids by female pigs. 2. Digestibility of protein and amino acids, and nitrogen metabolism during fattening. Archiv fur Tierernahrung 32(7/8): 465-476, 1982