EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

The partial purification and characterization of adenosine kinase from Entamoeba histolytica


The partial purification and characterization of adenosine kinase from Entamoeba histolytica



American Journal of Tropical Medicine and Hygiene 32(5): 976-979



ISSN/ISBN: 0002-9637

PMID: 6312825

Axenically grown Entamoeba histolytica was found to contain adenosine kinase. This organism lacks de novo purine biosynthetic pathways. Adenosine kinase provides the amoeba with a method for salvaging adenosine from ingested nucleosides or from degraded nucleotides. Adenosine kinase was purified 64-fold, by chromatography on Sephacryl S-200, DEAE-cellulose, and (C-8)-adenosine-agarose. The latter separated it from amebal adenylate kinase. Adenosine kinase has a molecular weight of 38,000 and requires glycerol for stability. It utilizes adenosine triphosphate to phosphorylate adenosine, and 7-deazaadenosine (tubercidin), but adenine 9-beta-D-arabinofuranoside (ara-A) is not detectably phosphorylated. It requires Mg++ as a cofactor.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 001269848

Download citation: RISBibTeXText

Related references

Partial purification and characterization of Entamoeba histolytica proteinases. Journal of Protozoology 23(2): 21A, 1976

Partial purification and characterization of ornithine decarboxylase from Entamoeba histolytica. International Journal for Parasitology 26(3): 253-260, 1996

Purification and partial characterization of an hemolytic activity from Entamoeba histolytica. Archives of Medical Research 23(2): 243-248, 1992

Partial purification and characterization of proteolytic enzymes in various strains of entamoeba histolytica. Molecular and Biochemical Parasitology (Suppl): 378, 1982

Partial purification and biochemical characterization of a soluble alpha-glucosidase from Entamoeba histolytica. FASEB Journal 15(5): A870, 2001

Partial purification and characterization of dolichol phosphate mannose synthase from Entamoeba histolytica. Glycobiology 10(12): 1311-1316, 2000

Isolation, purification, and partial characterization of an enterotoxin from extracts of Entamoeba histolytica trophozoites. Infection and Immunity 48(1): 211-218, 1985

Isolation, characterization and partial purification of a transferable membrane channel (amoebapore) produced by Entamoeba histolytica. Molecular and Biochemical Parasitology 33(3): 237-247, 1989

Characterization of Entamoeba histolytica adenosine 5'-phosphosulfate (APS) kinase; validation as a target and provision of leads for the development of new drugs against amoebiasis. Plos Neglected Tropical Diseases 13(8): E0007633, 2019

Ultrastructural comparisons between different species of entamoeba with tetranucleate cysts entamoeba moshkovskii entamoeba histolytica laredo strain entamoeba histolytica entamoeba invadens inst electron microscope. Bollettino dell'Istituto Sieroterapico Milanese 46(11-12): 583-595, 1967

Partial purification of the Entamoeba histolytica antigen characteristic for amoebiasis. Yonsei Reports on Tropical Medicine 7(1): 103-104, 1976

Isolation and partial purification of a surface glycoconjugate of Entamoeba histolytica. Molecular and Biochemical Parasitology 501: 127-138, 1992

Cysteine proteinase of Entamoeba histolytica. I. Partial purification and action on different enzymes. Molecular and Biochemical Parasitology 18(1): 103-112, 1986

Isolation and partial purification of a phosphorylated lipid containing glycoconjugate of entamoeba histolytica. Clinical Research 39(2): 244A, 1991

Partial purification of acid phosphomonoesterase in axenically grown Entamoeba histolytica NIH-200. Journal of Communicable Diseases 24(1): 42-45, 1992