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The primary processes of photosystem II in purified guard-cell protoplasts and mesophyll-cell protoplasts from Commelina communis L


, : The primary processes of photosystem II in purified guard-cell protoplasts and mesophyll-cell protoplasts from Commelina communis L. Planta 159(6): 554-560

Guard-cell protoplasts were isolated by enzymic digestion of the epidermis peeled from the abaxial surface of leaves from C. communis L. The protoplasts were separated from mesophyll-cell protoplasts and other contaminants by density-gradient centrifugation, and the purity of the preparations carefully and quantitatively assessed by light microscopy. The preparations of guard-cell protoplasts were then compared with mesophyll-cell protoplasts in terms of the activity of photosystem II as assessed by the light-induced evolution of O2 under both steady-state and flashing light and the characteristics of photosystem-II chlorophyll fluorescence. In all experiments, clear photosystem-II activity was found in guard-cell protoplasts, although some subtle distinctions between guard-cell and mesophyll-cell protoplasts were found. The contribution of any contamination by mesophyll-cell chlorophyll to guard-cell-protoplast signals was estimated to be less than 3% in all cases. Photosystem II is apparently present and active in guard cells of Commelina.

Accession: 001270500

PMID: 24258332

DOI: 10.1007/BF00409145

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Hipkins M.F.; Fitzsimons P.J.; Weyers J.D.B., 1983: The primary processes of photosystem ii in purified guard cell protoplasts and mesophyll cell protoplasts from commelina communis. Guard-cell protoplasts were isolated by enzymic digestion of the epidermis peeled from the abaxial surface of leaves from C. communis L. The protoplasts were separated from mesophyll-cell protoplasts and other contaminants by density-gradient cent...

Birkenhead, K.W.llmer, C., 1986: Some biochemical characteristics of guard cell and mesophyll cell protoplasts from Commelina communis L. Guard cell and mesophyll cell protoplasts of Commelina communis L., were isolated and used to investigate their various biochemical characteristics. Contamination of the samples by other cell types was very low and viability of the protoplasts, as...

Birkenhead K.; Willmer C.M., 1986: Some biochemical characteristics of guard cell and mesophyll cell protoplasts from commelina communis. Guard cell and mesophyll cell protoplasts of Commelina communis L., were isolated and used to investigate their various biochemical characteristics. Contamination of the samples by other cell types was very low and viability of the protoplasts, as...

Gautier, H.; Vavasseur, A.; Gans, P.; Lascève, G., 1991: Relationship between Respiration and Photosynthesis in Guard Cell and Mesophyll Cell Protoplasts of Commelina communis L. A mass spectrometric method combining (16)O/(18)O and (12)C/(13)C isotopes was used to quantify the unidirectional fluxes of O(2) and CO(2) during a dark to light transition for guard cell protoplasts and mesophyll cell protoplasts of Commelina co...

Lee, J.; Bowling, D.J.F., 1993: The effect of a mesophyll factor on the swelling of guard cell protoplasts of Commelina communis L. The effect of white, red and blue light on the swelling of guard cell protoplasts of Commelina communis has been investigated. White light and blue light caused protoplasts to swell but red light had no effect. Mesophyll cells were isolated from l...

Donovan, N.; Gibb, E.; Donkin, M.E.; Martin, E.S., 1985: A comparison of the kinetic properties of phosphoenolpyruvate carboxylase from guard-cell and mesophyll-cell protoplasts of Commelina communis. Some kinetic properties of partially purified phosphoenolpyruvate carboxylase (PEPCase) from guard-cell and mesophyll-cell protoplasts of Commelina communis are described. The PEPCase activity inherent to each cell type was determined and the appa...

Pantoja, O.W.llmer, C., 1986: Pressure effects on membrane potentials of mesophyll cell protoplasts and epidermal cell protoplasts of Commelina communis L. Membrane potentials of epidermal cell protoplasts and mesophyll cell protoplasts of Commelina communis were measured when the protoplasts were immobilized in a suction micropipette. When zero suction was employed, membrane potentials of both proto...

Pantoja O.; Willmer C.M., 1986: Pressure effects on membrane potentials of mesophyll cell protoplasts and epidermal cell protoplasts of commelina communis. Membrane potentials of epidermal cell protoplasts and mesophyll cell protoplasts of Commelina communis were measured when the protoplasts were immobilized in a suction micropipette. When zero suction was employed, membrane potentials of both proto...

Gautier, H.; Vavasseur, A.; Lascève, G.; Boudet, A.M., 1992: Redox Processes in the Blue Light Response of Guard Cell Protoplasts of Commelina communis L. Guard cell protoplasts from Commelina communis L. illuminated with red light responded to a blue light pulse by an H(+) extrusion which lasted for about 10 minutes. This proton extrusion was accompanied by an O(2) uptake with a 4H(+) to O(2) ratio...

Assmann S.M.; Moran N.; Schwartz A., 1988: Whole cell potassium currents in guard cell protoplasts gcp of vicia faba and commelina communis. Plant Physiology (Rockville) 86(4 SUPPL): 83