+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

The primary processes of photosystem II in purified guard-cell protoplasts and mesophyll-cell protoplasts from Commelina communis L

The primary processes of photosystem II in purified guard-cell protoplasts and mesophyll-cell protoplasts from Commelina communis L

Planta 159(6): 554-560

ISSN/ISBN: 0032-0935

PMID: 24258332

DOI: 10.1007/bf00409145

Guard-cell protoplasts were isolated by enzymic digestion of the epidermis peeled from the abaxial surface of leaves from C. communis L. The protoplasts were separated from mesophyll-cell protoplasts and other contaminants by density-gradient centrifugation, and the purity of the preparations carefully and quantitatively assessed by light microscopy. The preparations of guard-cell protoplasts were then compared with mesophyll-cell protoplasts in terms of the activity of photosystem II as assessed by the light-induced evolution of O2 under both steady-state and flashing light and the characteristics of photosystem-II chlorophyll fluorescence. In all experiments, clear photosystem-II activity was found in guard-cell protoplasts, although some subtle distinctions between guard-cell and mesophyll-cell protoplasts were found. The contribution of any contamination by mesophyll-cell chlorophyll to guard-cell-protoplast signals was estimated to be less than 3% in all cases. Photosystem II is apparently present and active in guard cells of Commelina.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 001270500

Download citation: RISBibTeXText

Related references

Some biochemical characteristics of guard cell and mesophyll cell protoplasts from Commelina communis L. Journal of experimental botany 37(174): 119-128, 1986

Relationship between Respiration and Photosynthesis in Guard Cell and Mesophyll Cell Protoplasts of Commelina communis L. Plant Physiology 95(2): 636-641, 1991

The effect of a mesophyll factor on the swelling of guard cell protoplasts of Commelina communis L. Journal of Plant Physiology 142(2): 203-207, 1993

A comparison of the kinetic properties of phosphoenolpyruvate carboxylase from guard-cell and mesophyll-cell protoplasts of Commelina communis. Planta 164(1): 115-120, 1985

Pressure effects on membrane potentials of mesophyll cell protoplasts and epidermal cell protoplasts of Commelina communis L. Journal of experimental botany 37(176): 315-320, 1986

Redox Processes in the Blue Light Response of Guard Cell Protoplasts of Commelina communis L. Plant Physiology 98(1): 34-38, 1992

Whole cell potassium currents in guard cell protoplasts gcp of vicia faba and commelina communis. Plant Physiology 86(4 Suppl): 83, 1988

Potassium ion uptake by swelling commelina communis guard cell protoplasts. Physiologia Plantarum 66(3): 469-475, 1986

Potassium ion uptake by swelling Commelina communis guard cell protoplasts. Physiologia Plantarum (København 1948) 66(3): 468-475, 1986

The effects of aba on protein synthesis in guard cell protoplasts of commelina communis. Plant Physiology 96(1 Suppl): 68, 1991

Responses of commelina communis l. guard cell protoplasts to abscisic acid. Journal of Experimental Botany 38(191): 992-1001, 1987

Carbon dioxide fixation by guard cell protoplasts of commelina communis. Journal of Experimental Botany 35(158): 1260-1264, 1984

Electrically Stimulated Fusion of Different Plant Cell Protoplasts : MESOPHYLL CELL AND GUARD CELL PROTOPLASTS OF VICIA FABA. Plant Physiology 67(4): 849-853, 1981

Effects of usnic acid on the oxygen exchange properties of mesophyll cell protoplasts from commelina communis l. Journal of Plant Physiology 139(1): 90-94, 1991

Volume changes of commelina communis guard cell protoplasts in response to potassium light and carbon dioxide. Physiologia Plantarum 66(3): 463-468, 1986