Transfer of Rhizobium meliloti nodulation genes to Sym+ and Sym- R. trifolii

Hirsch, A.M.; Drake, D.; Bang, M.

Plant Physiology 75(1, Suppl): 7


ISSN/ISBN: 0032-0889
Accession: 001275089

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Plasmid pRmJ30 is an 8.7 kb EcoR1 fragment of pRmSL26, which carries Rh. meliloti DNA sequences known to induce nodule development. Rh. trifolii carrying the symbiotic plasmid of Rh. meliloti (Sym+) and pRmJ30 was shown to elicit nodule development on all Trifolium pratense and T. repens roots; pRmJ30 or pRmSL26 introduced into Sym- Rh. trifolii failed to restore nodulation in both Trifolium species, and induced a very low frequency of white, ineffective nodules in lucerne.