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A comparison of methods for the high-performance liquid chromatographic and capillary gas-liquid chromatographic analysis of fatty acid esters



A comparison of methods for the high-performance liquid chromatographic and capillary gas-liquid chromatographic analysis of fatty acid esters



Journal of Chromatography 353: 319-328



A reversed-phase high-performance liquid chromatographic (RP-HPLC) method for the estimation of free fatty acids in plasma was compared with a method using capillary gas-liquid chromatography (GLC). The same extraction procedure was used for both methods. In the RP-HPLC method, the acids were separated as their anthrylmethyl esters on a C18 reversed-phase column, and detected by fluorescence. The coupling agent 2-bromo-1-methylpyridinium iodide was used with 9-(hydroxymethyl)anthracene.

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Accession: 001284163

Download citation: RISBibTeXText

PMID: 3700516

DOI: 10.1016/s0021-9673(01)87102-8



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